The chemiluminescence properties of firefly luciferin were studied, in a variety of chemical systems different from those in which it is usually studied. It was found that luciferin is a multifunctional chemiluminescence molecule, as it can behave as a chemiluminescence sensitizer, an emitter and a substrate. The first two roles are here reported for the first time, while we have found a novel ...

This paper reports a microchip with an integrated passive micromixer based on chaotic advection. The micromixer with staggered herringbone structures was used for luminol-peroxide chemiluminescence detection. The micromixer was examined to assess its suitability for chemiluminescence reaction. The relationship between the flow rate and the location of maximum chemiluminescence intensity was inv...

The spontaneous emission of chemiluminescence appears to reflect changes in the intracellular steady-state concentration of oxygen radicals. Stimulation of arachidonic acid metabolism in human platelets also induces chemiluminescence, which indicates the formation of highly reactive species by cyclooxygenase andlor lipooxygenase [1,2]. It was observed that arachidonic acid, linoleic acid and li...

A sensitive and simple method for measuring antioxidant activity was developed, based on photostoragechemiluminescence generated from photolyzed acridine and strong bases. This method was used to evaluate the antioxidant activity of food additives by measuring the inhibition of chemiluminescence at a gradient of antioxidant concentrations. Adipic, tartaric, citric, ascorbic, malic and fumaric a...

Blood phagocytes from patients with asthma have an increased capacity to produce reactive oxygen metabolites. We studied whether whole blood luminol-dependent chemiluminescence could detect this phenomenon in patients with a normal spirometry but bronchial hyperreactivity as determined with a methacholine bronchial challenge test. Whole blood chemiluminescence, serum eosinophilic cationic prote...

Light-emission of the perfused lung is induced by t-butyl hydroperoxide, giving chemiluminescence yields that oscillate between 800 and 1500 counts/s depending on the site and position of the lung. The response of the perfused lung to infusion with different hydroperoxides gives a pattern similar to that observed with the liver microsomal fraction; ethyl hydroperoxide shows a much higher chemil...

Chemiluminescent-based detection is entrenched throughout the biosciences today, such as in blotting, analyte and protein quantification and detection. While the biological applications of chemiluminescence are forever growing, the underlying principles of using a probe, an oxidizer and a catalyst (biological, organic or inorganic) have remained mostly unchanged for decades. Subsequently, chemi...

Total organic carbon (TOC) can reflect the total amount of matter in water. This paper introduces common methods measuring carbon, including high temperature combustion method, potassium persulfate oxidation spectrometry, ozone chemiluminescence supercritical water method and so on. At present, is most widely used for TOC measurement seawater, because its efficiency. sensor needs to be develope...

serum opsonic capacity against b. pertussis was studied by using quantitative chemiluminescence (cl), a method known to have several advantages over conventional methods in evaluating opsonization and phagocytosis. sera from unvaccinated infants was shown not to contain opsonins against b. pertussis and in unvaccinated infants suffering from whooping cough, no opsonins were detected. in adults ...