Monoclonal antibodies represent the most important group of protein-based biopharmaceuticals. During formulation, manufacturing, or storage, antibodies may suffer post-translational modifications altering their physical and chemical properties. Such induced conformational changes may lead to the formation of aggregates, which can not only reduce their efficiency but also be immunogenic. Therefo...

Introduction: C-reactive proteins (CRP) are a very good marker of inflammation and when the inflammation subsided, CRP quickly falls followed by erythrocyte sedimentation rate (ESR) thus it is a more sensitive and accurate reflection of the acute phase response than the ESR. As both alcoholic and infective hepatitis leads to inflammation in hepatocytes, CRP levels are increased in both conditio...

Methods for quantitative characterization of heterologous protein-protein interactions by means of analytical ultracentrifugation (AUC) include sedimentation equilibrium, tracer sedimentation equilibrium, sedimentation velocity, and analytical band sedimentation. Fundamental principles governing the behavior of macromolecules in a centrifugal field are summarized, and the application of these p...

Macromolecular sedimentation in inhomogeneous media is of great practical importance. Dynamic density gradients have a long tradition in analytical ultracentrifugation, and are frequently used in preparative ultracentrifugation. In this paper, a new theoretical model for sedimentation in inhomogeneous media is presented, based on finite element solutions of the Lamm equation with spatial and te...

The article is devoted to a problem of sedimentation of superfine aerosols with a characteristic size of particles, an order of values of micrometers. Acceleration of the sedimentation process is promoted by imposing of an acoustic field that leads to acceleration of coagulation of particles. Besides, pressure of the sound radiation directed down, accelerates gravitational sedimentation of an a...