The presence of PCR inhibitors in extracted DNA may interfere with the subsequent quantification and short tandem repeat (STR) reactions used in forensic genetic DNA typing. DNA extraction from fabric for forensic genetic purposes may be challenging due to the occasional presence of PCR inhibitors that may be co-extracted with the DNA. Using 120 forensic trace evidence samples consisting of var...

We describe the application of a simple, low-cost, and effective method of DNA extraction (hot sodium hydroxide and Tris, HotSHOT) to the diapausing propagules of continental aquatic invertebrates for its use in PCR amplification. We illustrate the use of the technique in cladocerans, rotifers, anostracans, notostracans, and copepod diapausing eggs. We compare the performance of the HotSHOT tec...

Bagi seorang muslim status halal suatu produk pangan maupun non-pangan harus terpenuhi secara mutlak, begitu juga dengan kosmetik. Kosmetik terdiri dari berbagai bahan dan yang menjadi titik kritis kehalalan kosmetik adalah kompleksitas komposisi memungkinkan penggunaan dasar berasal babi seperti gelatin, asam lemak, gliserin kolagen mana bahan-bahan tersebut sangat umum digunakan dalam pembuat...

The use of ion exchange beads is proposed as standards for the estimation of various elements in biological tissues. Elements to be detected or quantitated in biological tissues occur in three forms: as free ions or salts, in a crystalline phase, and bound to a matrix. For the latter category a standard system using ChelexlOO -jon exchange beads is proposed. Cations can be attached in a control...

BACKGROUND Early diagnosis and treatment reduces HIV-1-related mortality, morbidity and size of viral reservoirs in infants infected perinatally. Commercial molecular tests enable the early diagnosis of infection in infants but the high cost and low sensitivity with dried blood spots (DBS) limit their use in sub-Saharan Africa. OBJECTIVES To develop and validate a sensitive and cheap qualitat...

The shutdown of drinking water treatment plants (DWTPs) at the Langat River Basin, Malaysia, which provides to almost one-third population in basin, is very frequent, especially due chemical pollution river. This study explored sources based on eight specific intake points respective DWTPs suggest an integrated river basin management (IRBM). Analysis Al (250.26 ± 189.24 µg/L), As (1.65 0.93 Cd ...

INTRODUCTION: Certain mineral elements dismetabolism is known to play an important part in the pathogenesis of corneal degenerative and dystrophic diseases, as metalloenzymes participate connective tissues metabolism affecting their properties. Thus, keratoconus tissue depleted iron, copper zinc, what could be underlying cause cornea biomechanical properties impairment. Keratoconus modeling com...