The effects of constant exposure to high concentrations of methotrexate in vivo on the committed stem cell (CFU-C) were studied by in vitro culture of mouse bone marrow. Bone marrow samples were obstained from animals receiving a continuous infusion, and were cultured in a methotrexate-free semisolid gel system. The effects of methotrexate infusion on the pluripotent stem cell population (CFU-S...

The aim of this study was to assess the volume of airborne fungi in the indoor and outdoor environment of poultry and cattle houses in the Mazandaran Province in Iran. Indoor and outdoor air of twenty cattle houses and twenty-five poultry houses were sampled using a single-stage impactor, which draws air at 20 L min-1 and impacts sampled material onto Petri plates containing malt extract agar. ...

The aim of this study was to evaluate the multiplication S. aureus during whey powder production in laboratory scale. Whey inoculated with 106 CFU∙ mL-1 mixed culture at beginning processing and persistence microorganism after pasteurization, vacuum evaporation drying evaluated. recontamination by biofilms also Population 102 CFU∙mL-1 found evaporation. able multiply concentrated crystallizatio...

Normal human bone marrow contains cells that form granulocytic colonies in fibrin clot diffusion chambers implanted intraperitoneally in sublethally irradiated mice (CFU-d) A series of experiments was performed to determine the relationship between CFU-d and the cells that form colonies in agar culture in vitro (CFU-c). Low-density bone marrow cells ( < 1 070 g/cm3) were separated by velocity s...

The aim of the study is to compare counting of colony forming units (CFU), the time to positivity (TTP) assay and the molecular bacterial load (MBL) assay, and explore whether the last assays can detect a subpopulation which is unable to grown on solid media. CFU counting, TTP and the MBL assay were used to determine the mycobacterial load in matched lung samples of a murine tuberculosis model....

The biological target for interferon (IFN)a in chronic myeloid leukemia (CML) is unknown, but one possibility is that amplification of granulocyte-macrophage colony-forming cells (CFU-GM) is reduced. Replating CFU-GM colonies and observing secondary colony formation provides a measure of CFU-GM amplification. Amplification of CML, but not normal, CFU-GM in vitro was significantly inhibited by I...

In poultry industry, cross-contamination due to processing equipment and contact surfaces is very common. This study examined the extent of bacterial attachment to 6 different types and design of conveyor belts: stainless steel-single loop, stainless steel-balance weave, polyurethane with mono-polyester fabric, acetal, polypropylene mesh top, and polypropylene. Clean conveyor belts were immerse...

Sir, We have with interest read the recent article by Paterson et al. describing the prevalence of mecC-carrying methicillin-resistant Staphylococcus aureus (MRSA) in England. In that study, the prevalence of mecC MRSA was 0.45% of all MRSA. In contrast, in a recent study mecC-positive MRSA constituted 2.8% of all MRSA in Denmark in 2011. This difference may be caused by a different epidemiolog...

The impact of conservation additives, based on formic and propionic acids and bacterial-enzymatic preparations, on the number of mould fungi and yeast cells in silages was investigated. Silages were prepared from red clover (Trifolium pratense L.), Italian ryegrass (Lolium multiflorum L.) and their mixtures prepared at the ratio of 50:50. The aim of the experiment was to examine the influence o...

in this study, frozen yoghurt was produced as a synbiotic product. a food product containing both probiotics and prebiotics is named as synbiotic or functional food. lactobacillus acidophilus (la-5) as a probiotic bacteria was added to frozen yoghurt in two types; free and encapsulated, and its survivability was evaluated during 60 days storage at -18 °c . also fructo-oligosaccharide as a prebi...