نتایج جستجو برای: cfp 10
تعداد نتایج: 1019566 فیلتر نتایج به سال:
FRET is a light microscopy method for detecting protein-protein interactions within intact cells. The FRET protocol presented here is for CFP- and YFP-tagged proteins examined with an argon laser on a scanning confocal microscope. FRET is assayed by one of the most straightforward approaches available, namely, acceptor photobleaching. In this procedure, the YFP-tagged protein (the FRET "accepto...
In recent years diverse photolabeling techniques using green fluorescent protein (GFP)-like proteins have been reported1–7, including photoactivatable PA-GFP1, photoactivatable protein Kaede2, the DsRed ‘greening’ technique3 and kindling fluorescent proteins6,7. So far, only PA-GFP, which is monomeric and gives 100-fold fluorescence contrast, could be applied for protein tracking. Here we descr...
Abstract In this study, the production of bio-oil by pyrolysis with microwave (MW) heating was tested experimentally. Two magnetrons a total MW power 1,600 W were used in setup. The electric field strength 185.38 V·m −2 at an frequency 2.45 GHz. Cashew nut shells (CNS) or Cassia fistula pods (CFP) pyrolyzed 400°C, 500°C, 600°C, biomass-to-activated carbon ratio set 70:30, 80:20, 90:10. largest ...
INTRODUCTION Latent tuberculosis infection (LTBI) is detected with the tuberculin skin test (TST) before anti-TNF therapy. We aimed to investigate in vitro blood assays with TB-specific antigens (CFP-10, ESAT-6), in immune-mediated inflammatory diseases (IMID) for LTBI screening. PATIENTS AND METHODS Sixty-eight IMID patients with (n = 35) or without (n = 33) LTBI according to clinico-radiogr...
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