To cost-efficiently produce biofuels, new methods are needed to convert lignocellulosic biomass into fermentable sugars. One promising approach is to degrade biomass using cellulosomes, which are surface-displayed multicellulase-containing complexes present in cellulolytic Clostridium and Ruminococcus species. In this study we created cellulolytic strains of Bacillus subtilis that display one o...

This study investigated the activities of micro organisms involved in the degradation of organic matter in solid wastes and their potential to produce cellulolytic enzymes. Soil samples of decomposing waste piles were collected from four designated landfill sites in Lagos metropolis and analyzed for physicochemical properties, toxic heavy metal content and microbial populations. Findings reveal...

Effects of two microbial feed supplements on microbial activities in rumen-stimulating cultures and the rumens of steers fed a fescue hay-based roughage diet were evaluated. The yeast culture supplement contained Saccharomyces cerevisiae (1.4 to 4.2 x 10(9) colony-forming units [cfu]/g), whereas the mixed microbial supplement contained yeast, lactobacilli and enterococci (1.4 to 2.7 x 10(9) cfu...

In many ecosystems, global changes are likely to profoundly affect microorganisms. In Southern California, changes in precipitation and nitrogen deposition may influence the composition and functional potential of microbial communities and their resulting ability to degrade plant material. To test whether such environmental changes impact the distribution of functional groups involved in leaf l...

Cattle farming is one of the livestock business commodities in Indonesia. In production process, cattle farms produce waste form feces and leftover feed which can be processed such a way through decomposition process into organic fertilizer. The rate influenced by existing decomposer microbes. One microbes needed cellulolytic bacteria. research was conducted to obtain potential bacterial isolat...

An in vitro experiment was conducted to evaluate the effect of methylcellulose on the attachment of major cellulolytic bacteria on rice straw and its digestibility. Rice straw was incubated with ruminal mixture with or without 0.1% methylcellulose (MC). The attachment of F. succinogenes, R. flavefaciens and R. albus populations on rice straw was measured using real-time PCR with specific primer...

Bombyx mori L. (Lepidoptera: Bombycidae) have been domesticated and widely used for silk production. It feeds on mulberry leaves. Mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. Some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. Eleven isolates were obtained from the digestive tract of B. mori, including the Gram positiv...

Cellulolytic enzymes are synthesized by a large number of micro-organisms which include fungi, actinomycetes, gliding bacteria (myxobacteria) and true bacteria. However, only the fungi appear to excrete large amounts of cellulase enzymes in active form into culture media and not surprisingly these are the enzyme systems that have been most extensively studied. Bacterial cellulases are cell-wall...

The dominant rumen bacteria in high-arctic Svalbard reindeer were characterized, their population densities were estimated, and ruminal pH was determined in summer, when food quality and availability are good, and in winter, when they are poor. In summer the total cultured viable population density was (2.09 +/- 1.26) X 10(10) cells ml-1, whereas in winter it was (0.36 +/- 0.29) X 10(10) cells ...