The functional organisation of the bovine rumen epithelium has been examined by electron and light microscopy combined with immunocytochemistry in order to define a transport model for this epithelium. Expression of connexin-43, an integral component of gap-junctions, the tight-junction molecules claudin-1 and ZO-1 and the catalytic α-subunit of the Na-K/ATPase were demonstrated by SDS/PAGE and...

Extensive worldwide efforts are underway to produce knockout mice for each of the ~25,000 mouse genes, which may give new insights into the underlying pathophysiology of neurological disease. Microscopic magnetic resonance imaging (μMRI) is a key method for non-invasive morphological phenotyping, capable of producing high-resolution 3D images of ex-vivo brains, after fixation with an MR contras...

This trawl survey was carried out during 2013 for the stock assessment of families Haemulids, Nemipterids and Ariids in the Oman Sea. Sampling was carried out at five different stratum and depths. The highest value of CPUA of Haemulidae was estimated for Pomadasys stridens in “B” stratum (885.78 kg nm-2), for Pomadasys kaakan at depths of 10-20 m  (330.35 kg nm-2), and for Nemipteridae it was e...

OBJECTIVE(S) This study was carried out to evaluate the effect of maternal morphine exposure during gestational and lactation period on pyramidal neurons of hippocampus in 18 and 32 day mice offspring. MATERIALS AND METHODS Thirty female mice were randomly allocated into cases and controls. In case group, animals received morphine sulfate 10 mg/kg.body weight intraperitoneally during 7 days b...

Dopaminergic cells in the retina express the receptor for brain-derived neurotrophic factor (BDNF) (). To investigate whether BDNF can influence the development of the retinal dopaminergic pathway, we performed intraocular injections of BDNF during the second or third postnatal week and visualized the dopaminergic system with tyrosine hydroxylase (TH) immunohistochemistry. Both regimens of BDNF...

We establish a Harder–Narasimhan formalism for modifications of $G$ -bundles on the Fargues–Fontaine curve. The semi-stable stratum associated stratification ${B^+_{{\rm dR}}}$ -Grassmannian coincides with variant weakly admissible locus defined by Viehmann, and its classical points agree those basic Newton stratum. When restricted to minuscule affine Schubert cells, corresponds Dat, Orlik Rapo...

Monocarboxylate-H+ cotransporters, such as monocarboxylate transporter (MCT) SLC16A, have been suggested to mediate transruminal fluxes of short-chain fatty acids, ketone bodies, and lactate. Using an RT-PCR approach, we demonstrate expression of MCT1 (SLC16A1) and MCT2 (SLC16A7) mRNA in isolated bovine rumen epithelium. cDNA sequence from these PCR products combined with overlapping expressed ...

The expression of the voltage-gated K(+)-channel subunit Kv3.1b in the developing hippocampus was determined by immunoblot and immunohistochemical techniques. Kv3.1b protein was detected first at postnatal day (P) 8. The Kv3.1b-immunopositive cell number per tissue section reached a maximum at P14 and was maintained through P40. In contrast, the Kv3.1b protein content of isolated membrane vesic...

Skin constitutes an excellent barrier for the transdermal delivery of hydrophilic or high molecular weight drugs. Low efficacy demonstrated in clinical trials for some topical drugs is mainly due to low skin penetration ability related to the efficient barrier properties of stratum corneum (SC). Transdermal delivery of molecules in the skin is limited by the full epidermis, not just stratum cor...