Methods Here we described for the first time a non-viral mediated approach to reprogram primary human T cells by disruption of PD-1. Results We showed that the gene knockout of PD-1 by electroporation of plasmids encoding sgRNA and Cas9 was technically feasible. The disruption of PD-1 resulted in significant reduction of PD-1 expression but didn’t affect the viability of primary human T cells. ...

background: among all common techniques in sitedirectedmutagenesis, λ red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. in thismethod, there is always the risk of dna linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. objectives:to overcome this, we constructeda recombinant vector to disrupt phop gene in salmonella...

Recent studies have confirmed that lung microvascular endothelial injury plays a critical role in the pathophysiology of COVID-19. Our group and others demonstrated beneficial effects H2S several pathological processes provided rationale for considering therapeutic implications COVID-19 therapy. Here, we evaluated effect slow-releasing donor, GYY4137, on barrier function cell monolayer vitro, a...

Disruption of neutrophils causes the release of a cytoplasmic protein which can inhibit the growth of Candida albicans without killing the organisms. The present study was undertaken with a combined system in which candidacidal and candidastatic activities of human neutrophils could be compared. In this system it was found that disruption of either half or all of the neutrophils in the samples ...

Cost-effective microbial biosynthesis of 2?-fucosyllactose (2?-FL) allows its application in infant formula. The specific effects 2?-FL on the gastrointestinal immune barrier are still largely unknown. Here, we quantified and compared HMOs isolated from human milk, 2?-FL/lactose (Lac), expression mucus associated genes MUC2, TFF3, RETLB Golgi sulfotransferases, CHST5, GAL3ST5, goblet cells. We ...

Fetal androgen disruption, induced by the administration of anti-androgen flutamide (0.4, 2, and 10 mg/kg day) causes a long-term apoptosis in testicular germ cells in adult male rat offspring. One of the questions raised by this observation is the role of the Sertoli cells in the adult germ cell apoptotic process. It is shown here that Sertoli cells originating from 15-day-old rats treated in ...

Abstract This study is the first to investigate effects of tebuconazole (TEB) on physiological functions bovine testicular cells and epididymal spermatozoa. Motility plasma membrane integrity spermatozoa exposed TEB (0.001–100 µM) were evaluated at different incubation times (0–6 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Testicular cells, obtained from pare...

Laboratory experiments identified microviridin J as the source of a fatal molting disruption in Daphnia species organisms feeding on Microcystis cells. The molting disruption was presumably linked to the inhibitory effect of microviridin J on daphnid proteases, suggesting that hundreds of further cyanobacterial protease inhibitors must be considered potentially toxic to zooplankton.

Loss of epithelial cell polarity and inflammation are hallmarks of breast cancer development. Although the association between the disruption of tissue polarity and inflammation has been demonstrated, we know little about how these two events are coupled. Using the 3D co-culture model of mammary epithelial cells and monocytes, a recent study reveals a link between disruption of epithelial polar...