Purpose: To investigate the therapeutic effect and underlying mechanism of hypaphorine in a cellular model non-alcoholic fatty liver disease (NAFLD).Methods: Palmitic acid (PA) was used to induce NAFLD phenotype hepatocytes. Cell viability apoptosis were evaluated by CCK-8 flow cytometry assays. Inflammatory response measured enzyme-linked immunosorbent assay (ELISA). The on lipid accumulation ...

ABSTRACT Apoptosis and inflammation were the main hallmarks of sepsis-induced cardiomyopathy (SIC). Yes-associated protein isoform 1 (Yap1) miR-484 involved in mitochondrial fission apoptosis, especially proapoptotic roles SIC. Here, we investigated role Yap1 lipopolysaccharide (LPS)-treated H9c2 cells. was downregulated, while elevated by LPS treatment. Cell counting kit-8, flow cytometry, wes...

The sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. Tests evaluated included the U.S. Department of Agriculture (USDA) card test with 8% cell concentration (8%Card), USDA rapid automated presumptive test (RAP), Mexican rose bengal plate tests with 8 and 3% cell concentrations (8%RB and 3%RB), French rose bengal plate test with 4.5% cell co...

Anti-TNF inhibitors exert their therapeutic effect by inhibition of the excessive amounts TNF-? within body. Recombinant should be produced in a soluble refolded form to investigate effectiveness and efficiency anti-TNF-? compounds. In this research, designed cassette was subcloned pET28a expression vector expressed E. coli BL21 (DE3). The identity protein confirmed through SDS-PAGE Western blo...

Background: It has been proven that the antihypertensive agent nifedipine can cause gingival overgrowth as a side effect. The aim of this study was to analyze effects pharmacological treatment with on human fibroblasts activity, investigating possible pathogenetic mechanisms lead onset enlargement. Methods: expression profile 57 genes belonging “Extracellular Matrix and Adhesion Molecules” path...

OBJECTIVE To compare performance of contemporary aquaporin-4-immunoglobulin (Ig) G assays in clinical service. METHODS Sera from neurologic patients (4 groups) and controls were tested initially by service ELISA (recombinant human aquaporin-4, M1 isoform) and then by cell-based fluorescence assays: fixed (CBA, M1-aquaporin-4, observer-scored) and live (fluorescence-activated cell sorting [FAC...

VlsE is a 35-kDa surface-exposed lipoprotein of Borrelia burgdorferi that was shown previously to undergo antigenic variation through segmental recombination of silent vls cassettes with vlsE during experimental mouse infections. Previous data had indicated that sera from North American Lyme disease patients and experimentally infected animals contained antibodies reactive with VlsE. In this st...

We developed and characterized a stable Vero cell line constitutively expressing Peste des petits ruminants virus (PPRV) hemagglutinin (H) protein and assessed its potential use as diagnostic antigen in enzyme-linked immunosorbent assay (ELISA). PPRV H gene of the vaccine strain (Sungri-96) was amplified by reverse transcription (RT)-PCR, cloned into a eukaryotic expression vector (pTarget), an...