interferon regulatory factor 7 (irf7) gene was cloned from a subtractive cdna library constructed with mrnas obtained from rainbow trout (oncorhynchus mykiss) macrophage cell line (rts-11). using expressed sequence tag clones of submitted irf7 amino acid sequences, specific primers were designed. results showed that irf7 cdna contains an orf of 1251 nucleotides that translates into a 416 residu...

The release of vast quantities of DNA sequence data by large-scale genome and expressed sequence tag (EST) projects underlines the necessity for the development of efficient and inexpensive ways to link sequence databases with temporal and spatial expression profiles. Here we demonstrate the power of linking cDNA sequence data (including EST sequences) with transcript profiles revealed by cDNA-...

administering growth hormone increases growth rate of cultured fish. the aim of this study was isolation and synthesize of persian sturgeon gh cdna. the total rna was extracted from pituitary gland of persian sturgeon and cdna was synthesized. the full-length cdna sequence of persian sturgeon contains a 645 nucleotide open reading frame, which encoding a 214 amino acid residues. the position of...

A mixture of tetradecamer oligodeoxyribonucleotides complementary to the codons specifying the carboxyl-terminal sequence, Ile-His-Pro-Phe-His, of angiotensin was chemically synthesized as two pools and used for the isolation of a cDNA clone specific for angiotensinogen from a cDNA bank of rat liver mRNA sequences. The two pools (oligo 1 and oligo 2), each containing 24 oligodeoxyribonucleotide...