The Radiological Research Accelerator Facility at Columbia University has recently added a UV microspot irradiator to a microbeam irradiation platform. This UV microspot irradiator applies multiphoton excitation at the focal point of an incident laser as the source for cell damage, and with this approach, a single cell within a 3D sample can be targeted and exposed to damaging UV. The UV micros...

in this research, anti-microbial activity of ethanolic extract of licorice root against food spoilage microorganisms (lactobacillus delbrueckii, leuconostocmesenteroides, candida krusei, saccharomyces cerevisiae) in an orange flavored carbonated soft drink was investigated. the mic and mbc of extract against lactobacillus delbrueckii was 0.7 mg/ml and 0.8 mg/ml respectively, and against leucono...

The occurrence of bacteria in the food processing environments plays a key role in food contamination and development of spoilage. Species of the genus Pseudomonas are recognized as major food spoilers and the capability to actually determine spoilage can be species- as well as strain-dependent. In order to improve the taxonomic resolution of 16S rRNA gene amplicons, in this study we used oligo...

The microbial safety of foods continues to be a major concern to consumers, regulatory agencies and food industries throughout the world. Many food preservation strategies have been used traditionally for the control of microbial spoilage in foods but the contamination of food and spoilage by microorganisms is a problem yet to be controlled adequately. Although synthetic antimicrobials are appr...

The effect of a surface open to air on the ensiling process was investigated in 15 × 60 cm PVC silos in four trials, three with alfalfa (30, 41, and 49% dry matter, DM) and one with whole-plant corn (35% DM). Silos were sampled 1, 2, 5, 7, 14, and 28 d post-filling at 5, 20, 35, and 50 cm from the open surface. At 5 cm, little fermentation occurred as oxygen contents were at 10% or higher, and ...

Natural food antimicrobials are bioactive compounds that inhibit the growth of microorganisms involved in food spoilage or food-borne illness. However, stability issues result in degradation and loss of antimicrobial activity. Nanoencapsulation allows protection of antimicrobial food agents from unfavorable environmental conditions and incompatibilities. Encapsulation of food antimicrobials con...

Endopores (or spores), which are produced by certain bacteria, are capable of surviving food processing and causing food spoilage and foodborne illness. Dipicolinic acid (DPA), which constitutes about 10% of the spores’ dry weight, plays a major role on wet heat resistance of spores. Current methods like chromatography and fluorometry that are used to quantify DPA are time-consuming, expensive ...

Objectives: In the present study, Pediococcus acidilactici Ch-2 isolated from Chuli has been evaluated for its probiotic potential and its ability to produce bioactive compounds. Methodology: Isolation was done on MRS agar followed by the safety assessment of isolates. Isolates were screened on the basis of their antagonistic potential against pathogenic bacteria. Probiotic attributes of screen...