BACKGROUND The aim of this study was to investigate the transfection efficiency of cationic vectors (polyethylenimine; PEI25K and lipofectamine), electroporation and their combination in the human cancer cell lines, Raji human lymphoma and KB human oral carcinoma. MATERIALS AND METHODS Raji human lymphoma and KB human oral carcinoma cell lines were transfected with pcDNA3-CMV-Luc at various N...

BACKGROUND Currently available gene delivery vehicles have many limitations such as low gene delivery efficiency and high cytotoxicity. To overcome these drawbacks, we designed and synthesized two cationic lipids comprised of n-tetradecyl alcohol as the hydrophobic moiety, 3-hydrocarbon chain as the spacer, and different counterions (eg, hydrogen chloride [HCl] salt or trifluoroacetic acid [TFA...

the photocatalytic process using semiconductors with a nanostructure is one of the technologies usedfor the destructive oxidation of organic compounds such as surfactants. in this paper, thephotocatalytic degradation of cetyl pyridinium chloride (cpc), was investigated in aqueous phaseusing various semiconductors such as titanium dioxide (ti02), zinc oxide (zno), stannic oxide(sn02). the degrad...

the present study was aimed at evaluating the possible use of inter polymer complexed (ipc) films of xanthan gum (xg) and cationic guar gum (cgg) for formulating domperidone bioadhesive films. formation of bonds between –coo¯ groups of xg and –n + (ch 3 ) 3 groups of cgg was evident in the ftir spectra of ipc films. bioadhesive strength of the films was evaluated employing texture analyser. wat...

For systemic injection of cationic liposome/plasmid DNA (pDNA) complexes (cationic lipoplexes), polyethylene glycol (PEG)-modification (PEGylation) of lipoplexes can enhance their systemic stability. In this study, we examined whether intravenous injection of PEGylated cationic lipoplexes into tumor-bearing mice could deliver pDNA into tumor tissues and induce transgene expression. PEGylation o...

The silica-based Fe(III)-protoporphyrin and Zn-tetraphenylporphyrin stationary phases were examined for the HPLC separation of anions. The retention of nine common inorganic anions as well as benzoate anion (BA) and its hydroxy analogues (HBA) was examined using tartrate, acetate, and succinate eluents. The retention factors of inorganic anions on the FeProP stationary phase were in the order C...

Cationic gold nanoparticles offer intriguing opportunities as drug carriers and building blocks for self-assembled systems. Despite major progress on gold nanoparticle research in general, the synthesis of cationic gold particles larger than 5 nm remains a major challenge, although these species would give a significantly larger plasmonic response compared to smaller cationic gold nanoparticles...