Microbodies usually house catalase to decompose hydrogen peroxide generated within the organelle by the action of various oxidases. Here we have analyzed whether peroxisomes (i.e., catalase-containing microbodies) exist in Neurospora crassa. Three distinct catalase isoforms were identified by native catalase activity gels under various peroxisome-inducing conditions. Subcellular fractionation b...

Submit Manuscript | http://medcraveonline.com MOJ Toxicol (2016), 2(1): 00026 Abbreviations: RPM: Revolutions Per Minute; AST: Serum Aspartate Aminotransferase; ALT: Serum Glutamate Pyruvate Transaminase; Scr: Serum Creatinine; BUN: Blood Urea Nitrogen; C: Control; V: Venom; VC : Vitamin; VVC: Venom+Vitamin C ; TBS :Tris Buffered Saline; SOD: Superoxide Dismutase; Cat: Catalase; GSH-Px: Glutath...

Catalase, a ubiquitous heme enzyme, catalyzes conversion of hydrogen peroxide to water and molecular oxygen, protecting cells from oxidative stress. A C/T polymorphism in the promoter region of the CAT gene (rs1001179) affects transcriptional activity and RBC catalase levels. Oxidative stress may explain the observed increased postmenopausal breast cancer risk associated with hormone replacemen...

PURPOSE Retinal ischemia/reperfusion (I/R) injury results in the generation of reactive oxygen species (ROS). The aim of this study was to investigate whether delivery of the manganese superoxide dismutase gene (SOD2) or the catalase gene (CAT) could rescue the retinal vascular damage induced by I/R in mice. METHODS I/R injury to the retina was induced in mice by elevating intraocular pressur...

Preterm infants exposed to high concentration oxygen are prone to develop hyperoxic lung damage, which is an importan t underly ing cause of bronchopulmonary dysplasia (BPD).1 Although the causative agent for BPD has not been conclusively identified, hyperoxia-induced lung injury i s bel ieved to be a major factor. Data from both cellu lar and whole animal models suggest that hyperoxic lung dam...

The use of soil additives such as selenium can positively influence the antioxidative system plants, making them more tolerant to abiotic stresses. aim this work was evaluate concentration sodium selenite or selenate applied substrate that causes improve in plant growth and enzyme activities sunflower plants. treatments were divided in: control (absence selenium); 0.2 mg L-1 selenate; 0.4 0.8 s...

Lead has been linked to the development of hypertension via oxidative stress. Catalase plays an important role in the disposal of hydrogen peroxide in erythrocyte and its activity was determined by CAT gene. The aims of this study were to investigate (1) the association between blood levels of antioxidant markers such as catalase, superoxide dismutase, glutathione, glutathione peroxidase, oxida...

The effects of Ashwagandha root powder (0.7 and 1.4 g/kg body weight/day), administered for 15 and 30 days, was investigated on lipid peroxidation (LPO), superoxide dismutase (SOD) and catalase (CAT) activities in mice. While 15 days treatment with Ashwagandha root powder did not produce any significant change, 30 days treatment produced a significant decrease in LPO, and an increase in both SO...

As a safeguard against oxidative stress, the balance between the main antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) was believed to be more important than any single one, for example, dual-functional SOD/CAT enzyme has been proved to have better antioxidant ability than either single enzyme. By combining traditional fusion protein tec...

We had previously isolated a facultatively anaerobic hyperthermophilic archaeon, Pyrobaculum calidifontis strain VA1. Here, we found that strain VA1, when grown under aerobic conditions, harbors high catalase activity. The catalase was purified 91-fold from crude extracts and displayed a specific activity of 23,500 U/mg at 70 degrees C. The enzyme exhibited a K(m) value of 170 mM toward H(2)O(2...