Two simple and sensitive spectrophotometric methods (Method A and Method B) were developed for the estimation of Ethacridine lactate in pharmaceutical formulations. Method A is based on the oxidative coupling of Eathacridine Lactate with 3-methyl-2-benzothiazolinone hydrazone in the presence of Fe(III) to form a violet coloured chromoscme with a absorption maximum of 535nm. Method B is based on...

Many cholinesterase assays are performed to study the inhibition of cholinesterase (ChE) activity. Frequently a large number of samples are processed and Ellman's method [1] is the most commonly used [2,3]. Activity is estimated from the increment in absorbance between two reaction times when the reaction is not stopped. Bellino et al. [4] described a method based on Ellman's method whereby the...

The usefulness of determining protein-bound sialic acid in serum is obvious,because a significant increase in various serum sialoglycoprotein concentrations (haptoglobin, orosomucoid, a1-antitrypsin, and ceruloplasmin) has been observed in many pathological states (1, 2). We earlier described(3) a fully automated method in which thiobarbitunc acid is used as a reagent.This very sensitive method...

In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycerol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide. The hydrogen peroxide is monitored in the presence of horseradish peroxidase with 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone as th...

A simple micro (0.1 ml.) colorimetric method is described for the determination of serum calcium. This method employs the differential chelating characteristic of glyoxyl bis (2-hydroxyanil) in the presence of other divalent cations. The serum sample is placed in a colorimetric tube followed by additions of water, glyoxyl bis (2-hydroxyanil) reagent, and alkaline carbonate color developer. Chlo...

The question of whether or not there is an increase in adrenal cortical secretion during pregnancy remains unsettled. Morphologic studies of the adrenal during gestation have revealed enlargement of the gland in some species (1) and no enlargement in others (2). In the human female, several groups of investigators have observed a progressive rise in the plasma 17-hydroxycorticosteroid (17-OH-co...

For assaying plasma cholinesterase (EC 3.1.1.8) activity and phenotyping by means of dibucaine inhibition, we have compared a commercially available kit, in which butyrylthiocholine is used as substrate, with two reference methods, one using benzoylcholine and the other propionylthiocholine. With 50 different samples of three of the most common genetic variants, we could clearly differentiate t...

A direct colorimetric method was described for the rapid, sensitive and accurate determination of certain macrolide antibiotics; roxithromycin (ROX), azithromycin (AZM), and clarithromycin (CLM) in bulk powder and in pharmaceutical preparations. The proposed method is based on reaction of the studied drugs with haematoxylin reagent in the presence of boric acid to give a reddish-violet chromoge...

HE CHEMICAL ASSAY of diethylstilbestrol in extremely small amounts has not been previously accomplished in a satisfactory manner. The ultraviolet absorption of diethyistilbestrol has been proposed as a method (1), but this fails in the presence of other absorbing substances in tissue extracts. In addition, the absorption curve lacks any sharply defined band. Measurement of the phenolic function...

The glucose oxidase-peroxidase method for determining serum glucose, with the ammonium salt of 2,2’-azine-di (3 ethyl benzothiazoline-(6)-sulfonic acid) as the chromogen, was adapted to use with the single-channel AutoAnalyzer and the SMA 12/60 (Technicon Corp.). The extended linearity (up to 500 mg/100 ml on both systems) eliminated the need for numerous dilutions in the 300 to 500 mg/100 ml r...