DNA sequencing by synthesis (SBS) using reversible fluorescent nucleotide terminators is potentially an efficient approach to address the limitations of current DNA sequencing techniques. Here, we report the design and synthesis of a 3'-O-allyl photocleavable fluorescent nucleotide analogue, 3'-O-allyl-dGTP-PC-Bodipy-FL-510, as a reversible terminator for SBS. The nucleotide is efficiently inco...

We have discovered a mild, catalytic protocol for the regio- and stereoselective synthesis of trisubstituted allyl diarylphosphonates from the corresponding disubstituted allyl silyl ethers, circumventing the challenges related to the preparation and availability of stereodefined trisubstituted olefins. A closely related arylation reaction was also discovered during the methodology development....

Syrian petroleum coke, produced by a delayed coking unit, is characterized by a high percentage of fines which tends to reduce its commercial value. Blending coke fines using appropriate binders, such as bitumen and polyacrylic acid, can lead, however, to an improved coke that is similar in structure, appearance and other properties to better-quality coke lumps. In this work, samples of Syrian ...

The influence of various polymers on the release rate of lithium carbonate from matrix-type tablets was investigated in an attempt to formulate a sustained release solid dosage form. For this purpose, tablets containing 450 mg of lithium carbonate along with various amounts of Carbopol 934P, 971P, 974P, Pemulen and Eudragit RLPO as retarding agents and inactive ingredients (e.g. PVP, Avicel or ...

Drug delivery via buccal mucosa by means of buccoadhesive formulations offer distinct advantages over peroral administration. Recently, plant gums and exudates have been screened for their use as pharmaceutical adjuvants. The aim of this study is to evaluate matrix tablets containing Plantago psyllium seed mucilage in addition to carbopol as a mucoadhesive agent, and propranolol hydrochloride a...

(+)-SKF 10047 (N-allyl-normetazocine) is a prototypic and specific sigma-1 receptor agonist that has been used extensively to study the function of sigma-1 receptors. (+)-SKF 10047 inhibits K(+), Na(+) and Ca2+ channels via sigma-1 receptor activation. We found that (+)-SKF 10047 inhibited Na(V)1.2 and Na(V)1.4 channels independently of sigma-1 receptor activation. (+)-SKF 10047 equally inhibit...

Cross-metathesis (CM) has recently emerged as a viable strategy for protein modification. Here, efficient protein CM has been demonstrated through biomimetic chemical access to Se-allyl-selenocysteine (Seac), a metathesis-reactive amino acid substrate, via dehydroalanine. On-protein reaction kinetics reveal a rapid reaction with rate constants of Seac-mediated-CM comparable or superior to off-p...

In situ hydrolyzed acetals were coupled with in situ generated allyl boronates in a one-pot procedure, affording regio- and stereodefined homoallyl alcohols, epoxides and amino alcohols.

Thorough analyses of the photo- and devicephysics of poly-7-tert-butyl-1,3-pyrenylene (PPyr) by the means of absorption and photoluminescence emission, time resolved photoluminescence and photoinduced absorption spectroscopy as well as organic light emitting devices (OLEDs) are presented in this contribution. Thereby we find that this novel class of polymers shows deep blue light emission as re...

The aim of this study was to investigate the effects of polyacrylic acid polymers (Ultrez 10, Ultrez 20, Carbopol 980, and Carbopol 940) on the viscosity and the in vitro permeation of phenolic compounds from the gel prepared from natural horse chestnut seed extract. Experiments were performed in the presence and in the absence of peppermint oil (Mentha piperita). Our results showed that pepper...