We have investigated keratin interactions in vivo by sequentially extracting water-insoluble proteins from normal human epidermis with increasing concentrations of urea (2, 4, 6, and 9.5 M) and examining each extract by one- and two-dimensional gel electrophoresis, immunoblot analysis using monoclonal anti-keratin antibodies, and EM. The viable layers of normal human epidermis contain keratins ...

The epidermis is a stratified squamous epithelium composed primarily of keratinocytes that become postmitotic and undergo sequential changes in gene expression during terminal differentiation. The expression of the transcription factor CCAAT/enhancer binding protein beta (C/EBPbeta) within mouse epidermis and primary keratinocytes has recently been described; however, the function of C/EBPbeta ...

BACKGROUND Public Law 102-321 established a block grant for adults with "serious mental illness" (SMI) and required the Substance Abuse and Mental Health Services Administration (SAMHSA) to develop a method to estimate the prevalence of SMI. METHODS Three SMI screening scales were developed for possible use in the SAMHSA National Household Survey on Drug Abuse: the Composite International Dia...

BACKGROUND There is evidence that smoking is associated with poorer mental health. However, the underlying mechanisms for this remain unclear. We used longitudinal data to assess whether smoking uptake, or failed quit attempts, are associated with increased psychological distress. METHODS Data were used from Waves 3 (2004/05), 5 (2006/07) and 7 (2008/09) of the longitudinal New Zealand Survey...

deep cryogenic heat treatment is assumed as a supplementary heat treatment performed on steels before the finaltempering treatment to enhance the wear resistance and hardness of the steels. in this study, the effects of thedeep cryogenic heat treatment on the wear behavior and corrosion resistance of the 1.2080 tool steel were studiedusing the wear testing machine and polarization and impedance...

The application of conventional cutting fluids in machining operations is harmful and not eco-friendly. In this context, the present work focuses on environmental friendly cutting fluids such as vegetable oil based cutting fluids (VBCFs). The objective of this work is to determine the influence of vegetable oil based cutting fluids (sesame and coconut oil) with extreme pressure (EP) additive on...

The manufacturing of chemical vapour deposition (CVD) diamond coated shaft type cutting tools is demanding due to the complex design of the cutting edges and the cobalt content of the cemented carbide. The influencing parameters of substrate, pre-treatment and diamond film on the tool cutting performance are discussed. The optimised manufacturing route of CVD diamond coated thread milling drill...

The Al-alloying treated tungsten carbide (WC)-Co tool was subjected to grinding using a jaw crusher and planetary mill followed by three wet chemical treatment steps to establish an effective recycling process for WC scraps, especially those generated as bulky and hard scrap. This alloyed WC tool was readily ground to a powder of 1 mm or less and divided into two portions that were 150 μm in si...

BACKGROUND Empowerment programs have been shown to contribute to increased empowerment of individuals and build capacity within the community or workplace. To-date, the impact of empowerment programs has yet to be quantified in the published literature in this field. This study assessed the Indigenous-developed Family Wellbeing (FWB) program as an empowerment intervention for a child safety wor...

Silicon carbide is one of the most promising materials for power electronic devices capable of operating at extreme conditions. The widespread application of silicon carbide power devices is however limited by the presence of structural defects in silicon carbide epilayers. Our experiment demonstrates that optical second harmonic generation imaging represents a viable solution for characterizin...