The yeast species viz. Rhodotorula muciliginosa and Candida rugosa isolated from hydrocarbon contaminated sites were capable of producing biosurfactants in the presence of 2% (v/v) diesel as sole source of carbon and energy. The crude biosurfactants were purified using silica gel column chromatography followed by dialysis. Chemical structures of the purified biosurfactants were identified as di...

Enantioselective esterification of ibuprofen was conducted to evaluate the enzyme activity and ees of lipase from Candida rugosa (CRL7) in ten conventional organic solvents and three ionic liquids. Different alcohols were tested for selecting the most suitable acyl acceptor due to the fact that the structure of alcohols (branch and length of carbon chains; location of -OH functional group) coul...

This study investigated effect of virgin coconut oil (VCO)-derived fatty acids on the alteration lipid profiles and liver tissues high fat diet (HFD) fed mice. The initial mixture was successfully obtained via hydrolysis process using Candida rugosa lipase (CRL). Enrichment medium chain (MCFA) from acid distillation developed to achieve 3 fractions including Fraction I (MCFA mainly C8 C10), II ...

A core-shell structured Fe3O4-MCM-41 nanocomposite was prepared by means of a surfactant-directed sol-gel process. Candida rugosa lipase was then bound to the magnetic core-shell material by using glutaraldehyde as a cross-linking reagent. The as-prepared Fe3O4-MCM-41 support and the immobilized lipase were characterized in detail using enzyme activity assays, TEM, XRD, FTIR, VSM and nitrogen a...

In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers. Candida rugosa lipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe₃O₄ NPs) have been widely used for immobilizing enzyme...

This is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. Enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (AX) films. Different surface analytical techniques (FT-IR, TOF-SIMS, ESCA, CA) show that lipases from Mucor javanicus, Rhizopus oryzae and Candida rugosa successfully su...