Hierarchical systems that integrate nano- and macroscale structural elements can offer enhanced enzyme stability over traditional immobilization methods. Microparticles were synthesized using interfacial assembly of lipase B from Candida antarctica with (CLMP-N) and without (CLMP) nanoparticles around a cross-linked polymeric core, to characterize the influence of the hierarchical assembly on l...

One of the approaches to increasing the bioavailability of resveratrol is to protect its 3-OH phenolic group. In this work, regioselective acylation of resveratrol at 3-OH was achieved by transesterification with vinyl acetate catalyzed by immobilized lipase from Alcaligenes sp. (lipase QLG). The maximum yield of 3-O-acetylresveratrol was approximately 75%, as the lipase also catalyzes its furt...

A new assay was designed to measure the release of omega-3 acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid ( DHA)) from the hydrolysis of sardine oil by lipases immobilized inside porous supports. A biphasic system was used containing the fish oil dissolved in the organic phase and the immobilized lipase suspended in the aqueous phase. The assay was optimized by using a very active ...

In the present work, ability of different sodium-based salts like Na2CO3, Na2HPO4, Na2SO4 and Na2SO3 to salt out aqueous solutions two biodegradable non-ionic surfactants (Tergitol 15S7 Tergitol 15S9) has been demonstrated. We have empirically characterized immiscibility regions models Merchuk Othmer-Tobias equations were employed for binodal tie-line data, respectively. The effect phase formin...

High internal phase Pickering emulsion (Pickering HIPE) stabilized by enzyme-decorated metal-organic frameworks (MOFs) nanoparticles is developed for biphasic biocatalysts to enhance lipase catalysis and recycling. Specifically, enzyme decorated are prepared via ZIF-8 physisorption of a model Candida antarctica Lipase B (CALB), named ZIF-8@CALB, be both stabilizer catalytic sites. An oil-in-wat...