BACKGROUND Panicum hallii Vasey (Hall's panicgrass) is a compact, perennial C4 grass in the family Poaceae, which has potential to enable bioenergy research for switchgrass (Panicum virgatum L.). Unlike P. hallii, switchgrass has a large genome, allopolyploidy, self-incompatibility, a long life cycle, and large stature-all suboptimal traits for rapid genetics research. Herein we improved tissue...

Background and objectives:  Haplophyllum canaliculatum isan endemicand endangeredIranian plant from Rutaceae family. The object of this work was to study the volatile production in established shoot and callus cultures of Haplophyllum canaliculatum as well as isolation, identification and sequencing of 18S rRNA gene from callus culture. ...

Bourne (1944), describing the healing mechanism in tilt femoral defects, slated that initially they are filled by osteoid proliferation from the periosteum. Ma/.arow (I960) found that in the dog mandible the defect is tilled in from its cut surface and by bone trabeculae stemming chiefly from the periosteum. Mclchcr and Irving (1962) studied the healing mechanism in circumscribed defects in rat...

the present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of fritillaria imperialis l. there is no published method recommended for protoplast isolation and regeneration from fritillaria imperialis l. a range of factors, which influence the success of isolation and regeneration of f. imperialis protoplasts, were investigated. from the results obta...

Plants can be regenerated from various explants/tissues via de novo shoot meristem formation. Most of these regeneration pathways are indirect and involve callus Besides plant hormones, the role polyamines (PAs) has been implicated in processes. Interestingly, lateral root primordia (LRPs) Arabidopsis directly converted to meristems by exogenous cytokinin application. In this system, no formati...

The capacity of plants to regenerate is impressive. Plant cells can undergo somatic embryogenesis in culture, forming an embryo from a single cell or a group of somatic cells. In addition, plants exhibit de novo organogenesis, in which new organs and even entire plants are produced from other organs upon wounding. Almost as remarkable as the phenomenon of plant regeneration is how little we kno...

In the present study, small segments of cotyledon, apical bud, hypocotyl, and root of 10-day-old seedlings were isolated and transferred to Murashige and Skoog (MS) base medium with different treatments of phytohormones. Two media were used for apical bud culture: MS with indole-3-acetic acid (IAA) and kinetin (Kin) (1 mg/l) and the other medium, MS with double vitamin of MS and 6-benzylaminopu...

A rapid in vitro propagation system has been established from mature shoot tip and nodal segments of ahighly valuable medicinal plant Alternanthera sessilis (L.). The explants were cultured on Murashige andSkoog’s medium augmented with different concentrations and combinations of plant growth regulators forshoot bud initiation and multiplications. For shoot tip, highest freque...