The aim of this study was to synthesize by high shear homogenization method and to characterize new solid lipid nanoparticles loaded with green tea extract, nanoparticles which show high antioxidant activity. In this respect, cetyl palmitate and glyceryl stearate were used as solid lipids in combination with two nonionic surfactants, Tween20 and Tween80 and an ionic surfactant as lecithin. The ...

BACKGROUND Exposure to the fungal allergen Alternaria alternata as well as ryegrass pollen has been implicated in severe asthma symptoms during thunderstorms. We have previously shown that Alternaria extract induces innate type 2 lung inflammation in mice. We hypothesized that the innate eosinophilic response to Alternaria extract may enhance lung inflammation induced by ryegrass. METHODS Mic...

1. The effect of ultraviolet irradiation on acid-soluble and neutral-salt-soluble calf-skin collagen was studied by chromatography, gel filtration, amino acid analysis and sedimentation of the sub-units, and the reaction kinetics of degradation were obtained from viscosity and optical rotation measurements. 2. It was demonstrated that, whereas the structure of neutral-salt-soluble calf-skin col...

The relationship between adenosine deaminase (ADA) and a human membrane thymus leukemia (HTL) antigen-detected by an antithymocyte serum was explored. A freeze-thaw extract of the T-cell-derived cell line, MOLT 4, was applied to an immunoabsorbant column of a rabbit antiserum to calf ADA. The bound MOLT 4 ADA was eluted with 6 M urea. The recovered ADA had a specific activity of 490 mumol of ad...

In this study, n-butanol fraction (KBF) and aqueous fraction (KAF) isolated from methanol extract of Koelreuteria paniculata Laxm. leaves were evaluated for their antioxidant potential using (ABTS) 2, 2-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-2-picrylhydrazyl) assay, Reducing power assay and Superoxide anion radical scavenging assay. These fractions were also studi...

HeLa nuclear extract was resolved into a depleted fraction incapable of supporting mismatch repair in vitro, and repair activity was restored upon the addition of a purified fraction isolated from HeLa cells by in vitro complementation assay. The highly enriched complementing activity copurified with a DNA polymerase, and the most pure fraction contained DNA polymerase delta but was free of det...

To investigate factors involved in excision repair DNA synthesis, a soluble extract was prepared from permeable mouse sarcoma (SR-C3H/He) cells by homogenization and ultracentrifugation. DNA synthesis measured by using native calf thymus DNA as the template-primer and the extract as the polymerase source showed low activity. The DNA synthesis was enhanced more than ten-fold by the addition of a...