A new member of paromomycin group antibiotics has been isolated from the culture filtrate of Streptoverticillium sp. Al-R2827 which was isolated from a soil sample collected in India. In this report, the production, the isolation, characterization and structural elucidation of the antibiotic are reported. Production and Isolation Streptoverticillium sp. Al-R2827 was inocu-lated into a seed medi...

Background and Objective: The present study aimed to evaluate the optimum conditions for extracting and purifying penicillinase enzyme. The enzyme source was Bacillus licheniformis 6346 obtained from the Iranian Research Organization for Science and Technology. Materials and Methods: B. licheniformis was cultured in a medium containing mineral salts, nitrogen compounds, and cultured carbon sou...

BACKGROUND Epidural administration of dexamethasone might reduce postoperative pain in adults. We evaluated whether a caudal block of 0.1 mg kg(-1) dexamethasone combined with ropivacaine improves analgesic efficacy in children undergoing day-case orchiopexy. METHODS This randomized, double-blind study included 80 children aged 6 months to 5 yr who underwent day-case, unilateral orchiopexy. P...

b ackgro un d : the antimicrobial activity of silver nanoparticles has been investigated in medical fields in recent years, but there are few studies regarding its effect on oral microorganisms. the aim of the present study was to evaluate the in vitro antimicrobial and toxicity properties of nanosilver against two dental plaque microorganisms  and human gingival fibroblast (hgf) cell line. me ...

The present experiment was carried out to investigate the effects of exogenous adenosine 5'-triphosphate (ATP) and growth hormone (GH) on cellular H(+) efflux rate (extracellular acidification rate) and Ca(2+) concentration ([Ca(2+)](c)) in cloned bovine mammary epithelial cells (bMEC) raised from the mammary gland of a 26-day-pregnant Holstein heifer. Perifusion of 2-day cultured cells with a ...

BACKGROUND/AIMS Helicobacter pylori induces the apoptosis of gastric epithelial cells in vivo and in vitro. However, the molecular mechanism has not been clarified. The aim of this study was to investigate the effect of H pylori on the apoptosis of gastric epithelial cells and the expression of apoptosis related genes in vitro. METHODS Human gastric adenocarcinoma SGC-7901 cells were co-cultu...

We studied the artificial incubation method of red swamp crayfish Procambarus clarkii embryos using cell culture plates. In first experiment, cleavage stage or egg-nauplius were individually cultured at 20°C in well plates with different water volumes per [12 (4 ml), 24 (2 48 (1 ml) and 96 (0.25 ml)]. second 12-well 15, 20 25°C. Almost all died without hatching. Egg-nauplius survived to hatch. ...

A simple, precise and rapid stability-indicating ultra-performance liquid chromatography (UPLC) method is developed for the simultaneous quantitative determination of Telmisartan, Amlodipine besylate and Hydrochlorothiazide from their innovative poly pill combination drug product in the presence of degradation products. It involves a 100 mm x 2.1 mm, 1.7 μm C-18 column. The separation is achiev...

The objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aernginosa, Bacillus subtilis, Candida albicans, and one mixture of these were used. These strains were inoculated in brain heart infusio...