Alzheimer’s disease (AD) is an irreversible and progressive neurodegenerative disorder, characterized by β-amyloid plaques, neurofibrillary tangles loss of cholinergic neurons. Butyrylcholinesterase (BChE) inhibition one the most critical strategy for treatment AD since BChE causes inactivation neurotransmitter acetylcholine has positive effects on promoting formation fibrils. Our previous stud...

Dipsaci Radix has been proved to represent an effective treatment strategy for Alzheimer's disease (AD), but the potential active components in have not evaluated by AD-related bioassay. In this study, water fraction of had shown highest inhibitory effect on activity tests, including inhibition acetylcholinesterase (AChE), butyrylcholinesterase (BChE), β-site amyloid precursor protein cleaving ...

A rapid and economical method is reported for the preparation of an immobilized enzyme reactor (IMER) using silica-encapsulated equine butyrylcholinesterase (BuChE) as a model system. Peptide-mediated silica formation was used to encapsulate BuChE, directly immobilizing the enzyme within a commercial pre-packed column. The silica/enzyme nanocomposites form and attach simultaneously to the metal...

This work describes the sequential hydrolysis of bambuterol enantiomers and their monocarbamate metabolites (MONO) catalyzed by human butyrylcholinesterase (BChE) as well as the enzyme inhibition resulting from this process. Particular emphasis is given to the contribution given by MONO to the enzyme inhibition because it was not fully characterized in previous works. Bambuterol and MONO enanti...

Plasma pseudocholinesterase has no known physiological role but is essential for the rapid degradation of succinyicholine, a muscle relaxant used in surgical procedures. Depression of effective enzyme concentrations as a result of hepatocellular disease or exposure to anticholinesterase agents will increase the patient’s sensitivity to succinyicholine and prolong postoperative apnea. Synthesis ...

Due to the side effects of synthetic drugs, the therapeutic potential of natural products for Alzheimer's disease (AD) has gained interest. Morinda officinalis has demonstrated inhibitory effects on geriatric diseases, such as bone loss and osteoporosis. However, although AD is a geriatric disease, M. officinalis has not been evaluated in an AD bioassay. Therefore, M. officinalis extracts and f...

IV. CHARACTERIZATION OF CHOLINESTERASE ACTIVITY IN GREEN MUSSEL (Perna viridis) AS A POTENTIAL BIOMARKER IN MARINE BIOMONITORING 4.1. Abstract Green mussels (Perna viridis) play an important economic and ecological role in the coastal areas of Indonesia. P. viridis has been used as an eco-sentinel organism for marine biomonitoring programme in Asia regions. To magnify its competency as a releva...

A wide range of evidence shows that cholinesterase (ChE) inhibitors can interfere with the progression of Alzheimer's disease (AD). The earliest known ChE inhibitors, namely, physostigmine and tacrine, showed modest improvement in the cognitive function of AD patients. However, clinical studies show that physostigmine has poor oral activity, brain penetration and pharmacokinetic parameters, whi...