Immune mice which exhibited a delayed-type hypersensitivity reaction to bovine serum albumin after bovine serum albumin immunization and stimulation and normal mice that had been transferred with a lymphokine-rich fraction from the supernatant of concanavalin A-stimulated spleen cell cultures demonstrated resistance to Salmonella infection.

The dependence of nanoparticle concentration on the conformation of bovine serum albumin has been studied. A very small but consistent enhancement in the tryptophan emission is observed in the presence of sub-picomolar concentration of gold nanoparticles. At this concentration, the bovine serum albumin unfolding profile in presence and absence of nanoparticles are quite similar and the protein ...

Herein, we report the first systematic and unbiased evaluation of the BODIPY fluorophore library against a wide panel of biologically relevant molecules, and discoveries of 2 novel fluorescent probes for BSA and dopamine.

PURPOSE Recently it has been shown that the transport as well as clearance of retinol from isolated rod photoreceptors requires an extracellular factor. Interphotoreceptor retinoid-binding protein (IRBP) is a component of the interphotoreceptor matrix (IPM) and is known to bind visual cycle retinoids. Serum albumin and serum retinol-binding protein (sRBP), proteins capable of binding retinoids,...

Excess blood vessels are removed by apoptosis of endothelial cells, however, the signals responsible for this have not been defined. Apoptosis of cultured human umbilical vein endothelial cells is induced by deprivation of serum or adhesion. In this paper, apoptosis in human umbilical vein and microvascular endothelium was induced by deprivation of serum and or adhesion. Apoptosis was confirmed...

The objective of this study is to investigate the impact of Ultra-High Pressure (UHP) combined with moderate heat treatment on the allergenic properties of Bovine Serum Albumin (BSA). The effects of high pressure treatment at 100-500 MPa on allergenicity and structural properties of BSA were investigated. This study was performed using Competitive Indirect-Enzyme Linked Immunosorbent Analysis (...

The first paper of this series (1) described an electrophoretic demonstration of a complex between pepsin and bovine serum albumin during the course of the enzymatic hydrolysis of the latter. Several independent lines of evidence were presented that this complex is the Michaelis-Menten complex capable of being activated to give rise to reaction products. This communication reports the results o...

A quantitative method was developed for the determination of proteins in aqueous solution. Under optimal conditions, the linear ranges of calibration curves for the determination of bovine serum albumin (BSA), human serum albumin (HSA) and egg albumin (Alb) were 01.13 μg ml-1, 0-1.17μg ml-1 and 0-1.13μg ml-1, respectively. The detection limits were 15.74 ng ml-1 for BSA, 23.34 ng ml-1 for HSA a...