Abstract Single-cell RNA sequencing provides exciting opportunities to unbiasedly study hematopoiesis. However, our understanding of leukemogenesis was limited due the high individual differences. Integrated analyses hematopoiesis and potentially new insights. Here we analyzed ~200,000 single-cell transcriptomes bone marrow mononuclear cells (BMMCs) its subsets from 23 clinical samples. We cons...

Abstract Single-cell RNA-sequencing (scRNA-seq) has rapidly spread across multiple research fields, leading to new discoveries. Many applications of scRNA-seq are focused on cell type identification, gene regulatory networks, or biomarker discovery which require the interrogation specific sets well-characterized genes. In these cases, sequencing entire transcriptome may be adding unnecessary pr...

Abstract Background Activator protein-1 (AP1), a c-Fos–JUN transcription factor complex, mediates many cytobiological processes. c-Fos has been implicated in immunoglobulin (Ig)E activation of mast cells (MCs) via high-affinity IgE Fc receptor (Fc?RI) binding. This study examined involvement MC and tested the effects c-Fos/AP1 inhibitor T-5224 on MCs allergic responses. Methods In vitro studies...

The incidence of malignant melanoma is steadily rising. Due to its metastatic potential the cancer has a poor prognosis and responsible for 80% patients with skin dying. treatment was revolutionized by development new immunotherapies, however long-term responses are still limited few complete remissions rare due resistance therapy. Therefore, there need therapeutic options melanoma. Resistance ...

Immunoglobulin E (IgE) bound to multivalent antigen (Ag) elicits mast cell degranulation but not survival; on the contrary, IgE in the absence of Ag (IgE(-Ag)) induces survival only but not degranulation. Although these distinct responses are mediated through the same receptor, FcepsilonRI, the molecular mechanism generating the divergence is largely unknown. We recently showed that the signals...

The precise roles of mast cells in liver allograft rejection and tolerance are still unknown. This study aimed to explore the roles of mast cells in immune regulation and liver regeneration for tolerance induction by using rat models of orthotopic liver transplantation (OLT). Stem cell factor (SCF) and its receptor c-Kit, which are critical to the migration and development of not only stem cell...

T he largest experience with stem cell therapy in the heart has been with bone marrow mononuclear cells (BMMCs). Several single-center studies have shown modest, and in some cases transient, increases in ejection fraction (EF) following BMMC injection in patients with reperfused myocardial infarction. While this experience clearly demonstrated the safety of BMMC injection, the imaging readouts ...

Hydrogen (H2) is a major biodegradation product of implanted magnesium (Mg) alloys that are commonly used in the healing bone fractures. Our earlier study showed H2 can inhibit mouse marrow mononuclear cell (BMMC) osteoclastogenesis during differentiation these cells into osteoclasts, thereby facilitating fracture healing. However, way by which H2 inhibits remains to be el...