To study the immunomodulative activity caused by bovine leukemia virus envelope (BLV Env) peptide, sheep were immunized with two kinds of Th-epitope peptides, peptide 98 (BLV Env 98-117), and 61 (BLV Env 61-78). Four of eight immunized sheep showed specific proliferative responses against both of the peptide stimulations. To characterize the cells responding to the peptides, peptide-specific ce...

Effect of cadmium (Cd II) and lead (Pb II) was determined on cell proliferation, DNA synthesis, DNA repair, and DNA polymerase activity. The experiments were done on lymphocytes obtained from sheep infected with bovine leukaemia virus (BLV) and non-infected animals. All methods were based on [ H]-thymidine incorporation into DNA and performed in cell cultures stimulated by mitogens. Lymphocytes...

An immunoscreening strategy was used to isolate a cDNA clone encoding the binding domain for the external glycoprotein gp51 of the bovine leukemia virus (BLV). Three recombinant phages demonstrating BLV binding activity and containing 2.3-kbp cDNA inserts with identical nucleotide sequences were isolated from a lambda gt11 cDNA library of bovine kidney cells (MDBK). One clone, BLVRcp1, hybridiz...

in this study 19 blv infected and 2 healthy lymph nodes were analysed by western blotting to detect viral p 24 antigen . western blotting was carried out by rabbit anti p24 serum and p24 monoclonal antibody. rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kd bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kd bands. our results indic...

background and aims: bovine leukaemia virus (blv) is an oncogenic member of the genus deltaretrovirus of the family retroviridae. blv is the causative agent of enzootic bovine leukaemia and infects cattle worldwide, imposing economic impact on the dairy cattle industry. the purpose of this study was to estimate the seroprevalence of blv in cattle in some provinces of iran. materials and methods...

MIHAELA AURELIA TOMESCU University of Petroşani Department of Mathematics and Computer Science University Street 20, 332006 Petroşani ROMANIA [email protected] MIHAIL MEGAN Academy of Romanian Scientists Independenţei 54, 050094 Bucharest ROMANIA West University of Timişoara Department of Mathematics V. Pârvan Blv. No. 4, 300223 Timişoara ROMANIA [email protected] TRAIAN CEAUŞU West University o...

Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL ...

The etiology of sporadic bovine leukosis (SBL) is not known. Long-term monolayer cultures were made from calf (CLS), thymic (TLS) and skin (SLS) forms, and serological tests, electron microscopic observations and reverse transcriptase assays were employed for the detection of an etiological agent. Bovine leukosis virus (BLV) antigen and reverse transcriptase activities remained negative in cult...

Bovine papillomavirus (BPV) DNA sequences were detected in different tissues, in addition to epithelium. Cytogenetic abnormalities were observed in blood lymphocytes. The presence of more than one virus in a single tissue is a difficult aspect to evaluate, especially when the DNA sequences are detected in tissues that are not specifically targeted by the virus. BPV and bovine leukemia virus (BL...