Epstein-Barr virus (EBV) nonproducer Raji cells stably maintain approximately 45 copies of the EBV genome per cell, depending on the presence of the EBV-determined nuclear antigen 1 (EBNA-1) protein. We found that transfection of the EBV BZLF1 gene causes the disappearance of EBNA proteins on Western blots (immunoblots). On the basis of these results, we attempted to eliminate EBV plasmids in R...

No trials are currently conducted at SASEX to screen genotypes for resistance to ratoon stunting disease (RSD), a disease that is well established in most sugarcane growing regions of the world and considered to cause more yield losses worldwide than any other sugarcane disease. In South Africa, field trials have demonstrated that yield reductions under rainfed conditions can be as high as 40% ...

A novel and sensitive method has been developed to identify ciliary neuronotrophic factors (CNTFs) from tissue extracts after blotting to nitrocellulose paper. The CNTF proteins are required for the in vitro survival of embryonic chick ciliary ganglionic neurons. Tissue extracts containing such CNTFs are electrophoresed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfe...

Nine Scottish Borrelia burgdorferi isolates were investigated in IgG Western blot tests. Sera previously found to be positive and negative when tested by routine Western blots prepared from reference strain B. burgdorferi sensu stricto antigen had different outcomes with these isolates. Two isolates, E5 (Borrelia afzelii) and G4 (B. burgdorferi sensu stricto) performed well, reproducing Western...

A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and...

Two lysosomal proenzymes, procathepsins L and D, bind to mouse fibroblast microsomal membranes at acidic pH. This membrane association is independent of the mannose-6-phosphate receptors and requires the presence of the N-terminal propeptides of the enzymes. We have identified the protein that specifically binds procathepsin L at pH 5. A 43-kDa membrane protein coimmunoprecipitated with procath...

A cDNA clone corresponding to a novel cGMP phosphodiesterase inhibitory subunit (PDE gamma) was isolated from a bovine retinal cDNA library using a rod PDE gamma cDNA as a probe under conditions of reduced stringency. This new clone encodes an 83-amino acid protein approximately 80% identical to rod PDE gamma. The transcript for this protein is retina specific and 940 base pairs in length. Sout...

Genomic fragments coding for sequences expressed as abundant mRNA in female Drosophila melanogaster were isolated from a lambda library. Hybridization of these clones to polytene chromosomes. in situ, identified four which mapped to X chromosomal region 9A to 9B, the locus for yolk proteins 1 and 2 (Ypl,2) and two which mapped to 12A6-7 to 12D3, the locus for Yp3. These clones were mapped with ...

Distinct organellar forms of the beta-oxidation enzyme enoyl-coenzyme A (CoA) hydratase were partially purified and characterized from 2-day germinated pea (Pisum sativum L.) seedlings. The purification was accomplished by disruption of purified mitochondria or peroxisomes, (NH(4))(2)SO(4) fractionation, and gel permeation chromatography using a column of Sephacryl S-300. The organellar isozyme...

Figure 4 in the published article shows the same Western blot for two different ages of mice in error, in Fig. 4A and Fig. 4F. The representative blot for IRS in Fig. 4A (third from top) and that for pIRS Tyr in Fig. 4F (first from top) are the same images. The pIRS Tyr in Fig. 4F (first from top) needs to be replaced, and the authors have provided a corrected figure here, as well as the raw bl...