Objective: Blood culture (BC) is gold standard for diagnosis of fungemia. Contamination BC a major problem worldwide, since it may delay actual diagnosis. The aim this study was to evaluate “contaminant” vials with prolonged incubation (max 30 days) antimicrobial supplemented media observe any mycological growth. Materials and Methods: Routine BCs optained patients XXX Hospital year period were...

Inability to rely on macroscopic examination as an aid in identifying positive blood cultures was encountered when a hypertonic medium containing sorbitol was tested in a comparative study with an isotonic blood culture medium.

INTRODUCTION Bloodstream infection (BSI) is a leading cause of mortality in critically ill patients. The mortality directly attributable to BSI has been estimated to be around 16% and 40% in general hospital population and Intensive Care Unit (ICU) population, respectively. The detection rate of these infections increases with the number of blood samples obtained for culture. The newer continuo...

BACKGROUND Culture of S. Typhi is necessary for the definitive diagnosis of typhoid fever and provides isolates for antibiotic susceptibility testing and epidemiological studies. However, current methods are not fully optimised and sourcing culture media and bottles for culture media may be problematic. METHODOLOGY In two hospital laboratories in Viet Nam, comparisons of media for blood and s...

Introduction: Enteric fever continues to be a global health problem and isolation of organism from blood is the gold standard for the diagnosis. Objectives: Aim of this study was to find out the usefulness of three different methods such as blood culture, clot culture & Widal test for diagnosis of enteric fever. Materials & Methods: Total number of blood samples collected was 290 from patients ...

BACKGROUND Improved diagnostics for typhoid are needed; a typhoid controlled human infection model may accelerate their development and translation. Here, we evaluated a blood culture-PCR assay for detecting infection after controlled human infection with S. Typhi and compared test performance with optimally performed blood cultures. METHODOLOGY/PRINCIPAL FINDINGS Culture-PCR amplification of...

Over a 9-month period, 14 strains of Ralstonia pickettii were isolated from various biological samples inoculated in a blood culture medium. Molecular epidemiological investigation confirmed the relatedness of the strains. The source of the contamination proved to be the blood culture bottle caps.