Clinics Splenomegaly; chronic phase (lasts about 3 yrs) with maintained cell’s normal activities, followed by accelerated phase(s)(blasts still < 15%), and blast crisis (BC-CML) with blast cells > 30%; blood data: WBC: 100 X 10/l and more during chronic phase, with basophilia; a few blasts; thrombocytosis may be present; low leucocyte alkaline phosphatases; typical acute leukaemia (AL) blood da...

Dear Editor, Determination of the percentage of blasts in the bone marrow (BM) is one of the critical factors in diagnosing MDS. Flow cytometry (FCM) of BM cells has been introduced as an important co-criterion for diagnosing MDS [1-3]. However, FCM has not been well accepted because of the lack of consensus of the criteria to define a phenotypic myeloblast and the appropriate denominator for c...

Panel A (3400 magnification hematoxylin and eosin) of the BM biopsy shows extensive sheets (90%) of immature large cells in a background of small lymphoid cells (arrow). Increased proerythroblasts (.25% in the touch preparation) with irregular nuclei, prominent nucleoli, and basophilic vacuolated cytoplasm were observed. Panel B demonstrates a Wright-Giemsa–stained marrow aspirate smear showing...

Underwater blasts propagate further and injure more readily than equivalent air blasts. Development of effective personal protection and countermeasures, however, requires knowledge of the currently unknown human tolerance to underwater blast. Current guidelines for prevention of underwater blast injury are not based on any organized injury risk assessment, human data or experimental data. The ...

Background: This Cross sectional comparative study was performed to compare the mean fluorescence intensity of cluster of differentiation 45 between healthy individuals and patients with acute lymphoblastic leukemia. Patients and methods: Thirty three healthy individuals (mean age 11 years) and 41 patients with B cell acute lymphoblastic leukemia (mean age 7 years) were enrolled in the study...

The case is reported of a 45 year old woman with the rare leukaemia of natural killer cell large granular lymphocyte (NK/ LGL) type. Cytometric analysis of leukaemic blasts showed that they were positive for CD2, CD38, and CD56 antigens but negative for a series of antigens including CD3, CD7, CD16, and HLA-DR. Rearrangements of the beta T cell receptor, and heavy and kappa immunoglobulin genes...

Acute undiffentiated leukemia (AUL) is an acute leukemia with no more than one membrane marker of any given lineage. Blasts often express HLA-DR, CD34, and/or CD38 and may be positive for terminal deoxynucleotidyl transferase (TdT). The expression of CD34, HLA-DR, and CD38 has been shown in pro-T-ALL, although in this case, blasts should also express CD7 and cyCD3. However, some cases of T-ALL ...

DOI: 10.1590/S1679-45082016AI3516 Acute myeloid leukemia (AML) results from accumulation of abnormal blasts in the bone marrow, interfering with hematopoiesis.(1) Multiparametric flow cytometry is an important and well-known tool for AML diagnosis and for monitoring of minimal residual disease.(2) However, this technique is still poorly explored to elucidate clonal evolution of the leukemia. We...