Proteases have central roles in "life and death" processes due to their important ability to catalytically hydrolyze protein substrates, usually altering the function and/or activity of the target in the process. Knowledge of the substrate specificity of a protease should, in theory, dramatically improve the ability to predict target protein substrates. However, experimental identification and ...

Methyltransferases & epigenomics In recent years, we have witnessed greatly expanded attention to the enzymes that catalyze the transfer of methyl groups from S-adenosylmethionine (AdoMet) to DNA, RNA, proteins, lipids and small molecules [1]. The central role of methyltransferases in epigenetics was first realized with enzymes modifying DNA [2,3]; subsequent work has demonstrated the importanc...

We have developed a tool in order to identify sequences in relation to a sequence family database. This tool combines several algorithms: BLAST, multiple sequence alignment and phylogenetic tree building. After identification of the most similar gene family to the query sequence, this query sequence is added to the whole family alignment and the phylogenetic tree of the family is rebuilt includ...

Background: Human brucellosis is a zoonotic disease caused by Brucella melitensis, Brucella abortus, and Brucella suis. Brucella causes a chronic disease, which subverts the immune defense system of their hosts. In this study, the prevalence of an important Brucella virulence determinant, PrpA, which can modulate immune response, was determined in human isolates. Methods: Polymerase chain reac...

Classification of MHC molecules into supertypes in terms of peptide-binding specificities is an important issue, with direct implications for the development of epitope-based vaccines with wide population coverage. In view of extremely high MHC polymorphism (948 class I and 633 class II HLA alleles) the experimental solution of this task is presently impossible. In this study, we describe a bio...

Many bioinformatic applications are computationally intensive. While many suggested using multiprocessor machines or distributed processing to handle computationally intensive programs, porting existing applications to these architectures and managing these applications are in general difficult. This paper proposes a distributed server approach which adapts the concept of peer-to-peer communica...

Aim. The screening of molecular genetic markers for a minimally invasive assessment the radiation therapy effectiveness rectum malignant tumors. Materials and methods. study was carried out in 4 stages: 1) bioinformatic analysis TCGA (The Cancer Ge- nome Atlas) databases using GISTIC algorithm; 2) validation bioinformatics data model experiment on cell culture; 3) genes copy number features val...