Oil-polluted soil is one of the major sources biosurfactant producing bacteria. This study was to optimization, degreasing and de-staining potentials glycophospholipid produced by B. anthracis strain S62A isolated from spent engine oil-polluted soil. Five (5) bacterial isolates associated with oil – polluted primarily screened for production using emulsification index test, displacement surface...

today bleach and detergents are being frequently used and some people use their mixture for more cleaning. because of chemical interaction of bleach and detergent, chlorine gas was released and thereby it could be dangerous for human health. this study examined the effects of exposed toxic mixture of bleach and detergent on the mucosa layer and lamina mucosa conjunctiva in the mice. in this stu...

Amylolytic enzymes are most important in the biotechnology industries which are highly applied in food, fermentation, textile and paper industry. The enzyme is also used in starch liquefaction, brewing, pharmaceuticals, detergents, drugs, toxic waste removal and in oil drilling (Ajay & Fagade, 2003). In the present study isolation of a bacterial strain from soil samples was done and it's physio...

The cell surface antigen prominin-1 (alias CD133) has gained enormous interest in the past 2 decades and given rise to debates as to its utility as a biological stem and cancer stem cell marker. Important and yet often overlooked knowledge that is pertinent to its physiological function has been generated in other systems given its more general expression beyond primitive cells. This article br...

Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E. coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immunoaffinity purification yielded receptors at >90% purity. Secondary structure analysis using circular dichroism indicated that the purified receptors...

Membrane proteins are generally unstable in detergents. Therefore, biochemical and biophysical studies of membrane proteins in lipidic environments provides a near native-like environment suitable for membrane proteins. However, manipulation of proteins embedded in lipid bilayer has remained difficult. Methods such as nanodiscs and lipid cubic phase have been developed for easy manipulation of ...

The reversal of the inhibitory action of antiseptics by substances showing a specific affinity for the inhibiting agent has been reported for many compounds, the most striking effect probably being the reversal of the action of mercurials by H2S, glutathione, sodium thioglycolate, etc. Valko and DuBois (1944) have reported that the germicidal action of cationic detergents against both gram-posi...