Objectives—Occupational asthma is the principal cause of respiratory disease in the workplace. The enzymes phytase and â-glucanase are used in the agricultural industry to optimise the nutritional value of animal feeds. A relation between these enzymes and occupational asthma in a 43 year old man was suspected. Methods—Inhalation challenge tests were performed with the enzymes phytase, â-glucan...

Phosphorus (P) losses from pastures fertilized with poultry litter contribute to the degradation of surface water quality in the United States. Dietary modification and manure amendments may reduce potential P runoff losses from pastures. In the current study, broilers were fed a normal diet, phytase diet, high available phosphorus (HAP) corn diet, or HAP corn + phytase diet. Litter treatments ...

The current direct colorimetric assay for phytase activity in feeds has interference from high P background and other factors. Our objective was to develop a rapid and reliable spin column method to accurately determine phytase activity in feed ingredients or complete diets. After the feed sample was extracted by stirring in 0.2 M citrate buffer, pH 5.5, for 30 min at room temperature, the oily...

An experiment was carried out to evaluate effects of temperature, pH, and incubation time on the stability of intrinsic phytases. The experiment was conducted as a 3*4*3*3 factorial arrangement with 3 feed ingredients (rye, wheat, and barley), 4 temperatures (T) (20, 38, 55, and 80°C), 3 pH levels (3, 5.5, and 8), and 3 incubation times (30, 60, and 120 min), with 2 replicates per treatment. Af...

Environmental concerns about phosphorus (P) losses from animal agriculture have led to interest in dietary strategies to reduce the concentration and solubility of P in manures and litters. To address the effects of dietary available phosphorus (AvP), calcium (Ca), and phytase on P excretion in broilers, 18 dietary treatments were applied in a randomized complete block design to each of four re...

The objective was to produce breakfast flakes by extrusion using whole meal from sprouted wheat increase mineral bio-accessibility and provide intrinsic sweetness without compromising their textural properties. Resting the ingredient mixture containing (sprouted) at 50 °C pH 3.8 for 1 h prior flake production allowed in situ endogenous phytase action. This resulted end-products that had higher ...

Phytate is the primary storage form of phosphate in plants. Monogastric animals like poultry, pigs and fishes have very low or no phytase activities in their digestive tracts therefore, are incapable to efficiently utilize phytate phosphorus from the feed. Phytase from microbial sources are supplemented to feedstuff of these to increase the uptake of phytate phosphorus. In the present work effo...

This study presents a review on applications of phytase from thermophilic fungal isolate, Sporotrichum thermophile. Under optimization, improvement in phytase production can be achieved in both solid state (2-fold) and submerged (2.6-fold) fermentations. Phytase could also be produced by alginate-immobilized S. thermophile, and enzyme production was sustainable over 5 repeated cycles. Purified ...

BACKGROUND Although fungal phytase is frequently used as an additive to animal feed few investigations of its allergenic property have been conducted. METHODS Fifty-three subjects occupationally exposed to powdered phytase from Aspergillus niger were studied. Exposure data and symptoms were registered by the company physician. RESULTS Thirty-eight subjects complained of work-related respira...

Phytases are phosphohydrolytic enzymes that initiate stepwise removal of phosphate from phytate. Simple-stomached species such as swine, poultry, and fish require extrinsic phytase to digest phytate, the major form of phosphorus in plant-based feeds. Consequently, this enzyme is supplemented in these species' diets to decrease their phosphorus excretion, and it has emerged as one of the most ef...