the interaction between [pt(phen)(pyrr-dtc)]no3 (where phen = 1,10-phenanthroline and pyrr-dtc =pyrrolidinedithiocarbamat) with human serum albumin (hsa) was studied by fluorescence, uv–vis absorption, circular dichroism (cd) spectroscopy and molecular docking technique under like physiological condition in tris–hcl buffer solution at ph 7.4. uv-vis absorption spectroscopy indicates that the pro...

introduction genetic background has known to be associated with the outcome of human t cell lymphotropic virus (htlv) type i infection. in the present study we investigate the association between gm-csf gene polymorphisms with the outcome of htlv-i infection. materials and methods we analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating fa...

background: fascioliasis is a chronic hepatic disease and may be resulted from mechani­cal/molecular parasite adhesion to host liver tissue. the aim of this study was to detect surface car­bohydrate and lectin, carbohydrate-binding protein isolation that might be responsible of this molecular binding. methods: the present experimental work was conducted in the department of medical parasitol­og...

The interaction of human serum albumin with Ethyl 2-[2-(dimethylamino)-4-(4-nitrophenyl)- 1,3-thiazole-5-yl]-2-oxoacetate was investigated by using isothermal titration UV-visible spectrophotometry in tris-buffer, pH 7.4. According to these results, it was found that there are a set of 4 binding sites for this ligand on HSA with positive cooperativity in the binding process. This thiazole deriv...

Abstract We report the G protein-first mechanism for activation of protein-coupled receptors (GPCR) three closest subtypes opioid (OR), ?OR, ?OR and ?OR. find that they couple to inactive Gi protein-bound guanosine diphosphate (GDP) prior agonist binding. The protein forms anchors intracellular loops apo-?OR, apo-?OR apo-?OR, inducing opening cytoplasmic region form a pre-activated state holds ...

Aim. The idea of the study was to compare and generalize RCSB Protein Data Bank ChEMBL data in order establish structural biological relationship experimentaly proved effectors FtsZ with binding sites. Methods. Literature database search. Comparison protein ligand structures. structure modeling, MD, superimposition, etc. Results. experimental protein-ligand complexes structures bacterial were r...

Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating...

Purpose: To investigate the mechanism of action Danhong Injection (DHI) in chronic heart failure (CHF).
 Methods: Network pharmacology was employed to identify bioactive compounds and targets DHI CHF. Bioinformatics analysis used examine potential biological functions pathways candidate targets, while molecular docking conducted evaluate binding affinity ligand-protein complex.
 Resul...

Deoxyribonucleic acid (DNA) is an important molecular target for anti-cancer agents due to its involvement in gene expression and protein synthesis which are fundamental steps in cell division and growth. A number of antineoplastic agents interfere with DNA and hence disturb the cell cycle. Compounds including planar aromatic rings are privileged scaffolds in binding to the DNA. This characteri...