The vacuolar-type ATPase (H+ATPase) is a ubiquitously expressed multisubunit pump whose regulation is poorly understood. Its membrane-integral a-subunit is involved in proton translocation and in humans has four forms, a1-a4. This study investigated two naturally occurring point mutations in a4's COOH terminus that cause recessive distal renal tubular acidosis (dRTA), R807Q and G820R. Both lie ...

Background: Novel roles for the interaction of cardiotonic steroids to Na+/K+-ATPase have been established in recent years. The aim of the present study was to investigate the intracellular signaling events downstream the action of ouabain on Na+/K+-ATPase in Sertoli cell obtained from immature rats. Treatment of Sertoli cells with ouabain (1 μM) induced a rapid and transient increase in the ex...

OBJECTIVE This article aims to investigate the expression of vacuolar-H + -ATPase (V-ATPase) in non-small cell lung cancer (NSCLC) and its variations with pathological type and grade. Furthermore, to evaluate the chemotherapy drug sensitivity of different cancer tissues as well as its correlation with V-ATPase expression in NSCLC. METHODS V-ATPase expression was examined in 92 NSCLC tissue sa...

Objective: Noncoding RNAs are emerging as important players in gene regulation and cardiovascular diseases. Their roles the pathogenesis of atherosclerosis not fully understood. The purpose this study was to determine role played by a previously uncharacterized long noncoding RNA, RP11-728F11.4, development mechanisms which it acts. Approach Results: Expression microarray analysis revealed that...

Triton X-100 (TX-100) extraction of Spiruplasnta citvi plasma membrane solubilized two types of ATPase differing in their pH of maximum activity. The activity measured at pH 8.5 was inhibited by vanadate and the activity measured at pH 6-5 was not. The vanadate-sensitive ATPase had a relatively basic isoelectric point (8.65) and therefore could be separated from the vanadate-insensitive ATPase ...