Conformational changes accompanying the formation of binary and tightly bound ternary complexes of thymidylate synthetase and all possible combinations of three folate analogs (N-10-ethyl-quinazoline, folic acid triglutamate, and folic acid) and three deoxyribonucleotides (5-fluoro-2'-deoxyuridylic acid (FdUMP), 2'-deoxyuridylic acid (dUMP), and thymidylic acid (dTMP] were studied by means of u...

Studies on the mechanism of substrate interaction with rabbit muscle lactate dehydrogenase have recently been reported (1). These investigations, which were primarily kinetic in nature, indicated that nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide must add to the enzyme before the binding of substrates pyruvate and L-lactate. It was also noted that ternary compl...

the vo(iv) complexes of tridentate ono schiff base ligands derived from 2- aminobenzoicacid and salicylaldehyde derivatives were synthesized and characterized by ir, uv–vis and elemental analysis. electrochemical properties of the vanadyl complexes were investigated by cyclic voltammetry. a good linear correlation was observed between the oxidation potentials and the electron-withdrawing charac...

We report a novel 3D structure-based method of predicting protein-protein (PP) interaction (PPI) partners. The method involves screening for pairs of tetrahedra representing interacting amino acids at the interface of the PP complex, with one tetrahedron on each protomer*. H-bonds and VDW interactions at the interface of the two interacting protein molecules in the complex are first determined,...

The Co-ordination compounds bear an important role in analytical, bio and electro chemistry. In this paper, the formation constants of binary complexes Actinide metal ions Th (IV) UO2 (II) with vitamin-U (Methylmethionine Sulfonium Chloride) have been carried out at constant temperature 25 ± 0.1 °C ionic strength µ =0.2M using Irving–Rossotti method. Various factors influencing stabilities comp...

To facilitate studies of multicomponent protein complexes, I have developed an Escherichia coli expression system which coexpresses up to four polypeptides from a single plasmid. The modular nature of the system enables efficient subcloning of a gene into each of the 4 cassettes in the polycistronic expression vector. Restriction sites present in the polycistronic expression vector allow both a...

Understanding the function of the thousands of cellular proteins is a central question in molecular cell biology. As proteins are typically part of multiple dynamic and often overlapping macromolecular complexes exerting distinct functions, the identification of protein-protein interactions (PPI) and their assignment to specific complexes is a crucial but challenging task. We present a protein ...