Effect of an intraperitoneal injection of cimetidine at a daily dose of 160 mg/kg for two weeks on biliary lipid secretion, fecal excretion of bile acids and intestinal bile acids was studied in rats. Bile flow, biliary lipid secretion and fecal excretion of bile acids remained unchanged, while the pool size of bile acids decreased by cimetidine treatment. Chenodeoxycholic acid increased with a...

BACKGROUND Postoperative bile duct leak following hepatobiliary and pancreatic surgery can be intractable, and the postoperative course can be prolonged. However, if the site of the leak is in the distal bile duct in the main biliary tract, the therapeutic options may be limited. Injection of absolute ethanol into the bile duct requires correct identification of the bile duct, and balloon occlu...

Bile from Alligator mississippiensis was found to contain a mixture of more than twenty bile acids, bile alcohols, and neutral sterols. Bile acids and bile alcohols were purified by reversed-phase high performance liquid chromatography and thin-layer chromatography. Concentrations were measured by gas-liquid chromatography on 1% HiEFF-8BP and identifications were made by mass spectrometry. The ...

The molecular pathogenesis of cystic fibrosis (CF) liver disease is unknown. This study investigates its earliest pathophysiologic manifestations employing a mouse model carrying ∆F508, the commonest human CF mutation. We hypothesized that, if increased bile salt spillage into the colon occurs as in the human disease, this should lead to a hydrophobic bile salt profile and to “hyperbilirubinbil...

Purpose: Laparoscopic cholecystectomy (LC) has become the gold standard of management of gallstone disease. LC is associated with a two-to-four times higher incidence of bile duct injury, which is a rare but more serious complication than open cholecystectomy. We reviewed our experiences with the management of bile duct injury during laparoscopic cholecystectomy. Methods: From January 1999 to A...

Bile acids are steroidal amphipathic molecules derived from the catabolism of cholesterol. They modulate bile flow and lipid secretion, are essential for the absorption of dietary fats and vitamins, and have been implicated in the regulation of all the key enzymes involved in cholesterol homeostasis. Bile acids recirculate through the liver, bile ducts, small intestine and portal vein to form a...

3alpha-Hydroxysteroid dehydrogenase (3alpha-HSD) from Pseudomonas testosteronei and diaphorase (lipoyl dehydrogenase) from Clostridium spp were immobilized individually onto alkylamine glass beads through glutaraldehyde coupling. A cost-effective enzymic colorimetric method for determination of bile acid in the serum and bile was developed employing mixture of the immobilized enzymes. The metho...

INTRODUCTION: Bile acids, phospholipids, and cholesterol are the major lipid components in human bile [1]. The composition of bile is altered in various chronic cholestatic diseases and determining such alterations will be of great clinical importance in understanding the pathophysiology of these diseases [2]. Although there are a few chromatographic [3] and NMR-based methods [4,5] available fo...

Bile acids are endogenous molecules that originate from the liver and transport via bile to the intestines. They normally regulate cholesterol homeostasis, stimulate lipid solubilization and mediate metabolic signaling. Early studies implicated that disorders of bile acids compositions and concentrations can cause liver injury. Several hydrophobic bile acids are toxic and ample increases of the...

Obesity, diabetes, and metabolic syndromes are increasingly recognized as health concerns worldwide. Overnutrition and insulin resistance are the major causes of diabetic hyperglycemia and hyperlipidemia in humans. Studies in the past decade provide evidence that bile acids are not just biological detergents facilitating gut nutrient absorption, but also important metabolic regulators of glucos...