Astragalus species are medicinal plants which produce valuable secondary metabolites, especially cycloartane-type glycosides. In this study, stem and leaf explants of Astragalus trojanus were subjected to different plant growth regulators, environmental conditions and media compositions to identify their callus responses. Stem and leaf explants were cultured in Murashige and Skoog (MS) and wood...

Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal analysis to investigate how wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq analysis revealed that wounding induc...

Different explants from diploid and tetraploid plants of Arabidopsis thaliana ecotype Wilna were cultured in vitro on the same medium in the same conditions. The mode and capacity for regeneration and the ploidy level of regenerants were examined in eight callus lines. The explant responses to in vitro conditions depended on the ploidy level of donor plants and on the type of explant. Callus de...

Single cells were mechanically isolated from leaf-derived callus of mature Juniperus oxycedrus L. These cells divided and gave rise to callus when plated on medium containing growth regulators. Best plating efficiency was obtained on a modified Schenk and Hildebrandt medium supplemented with 0.6 micro M 2,4-dichlorophenoxyacetic acid and 100 mg l(-1) casein hydrolyzate. Although single-cell-der...

An efficient and reproducible protocol for Agrobacteriummediated transformation of an agronomically useful Oryza sativa variety, Pusa Basmati1 (IET10364) has been standardized. Although several reporters have been claimed the Agrobacteriummediated transformation but here we studied the effect of acetosyringone and age of callus on transformation frequency for scutellumderived callus of Pusa bas...

The present study is based on the in vitro production of callus, its long period maintenance and antibacterial potentiality. Callus induction and formation occurred by full strength MS media in combination with various concentration (0.5mg/l, 1.0mg/l, 1.5mg/l, 2.0mg/l) of 2, 4-D effective in combination with constant concentration of BA (2.5mg/l) and NAA (0.5mg/ml). The leaf calli were sub cult...

BACKGROUND AND PURPOSE Evaluation of distraction callus is important for determination of the optimal time for removal of external fixation. We attempted to determine whether there might be a relationship between the electrical impedance of bone and callus maturation, with a view to using impedance as a way of knowing when to remove a fixator. METHODS We applied an external lengthener to the ...

Seedling, seedling parts and callus cultures of onion were tested for their antidiabetic activity by feeding the tissue-extracts to diabetic rats. The results indicated much higher antidiabetic activity in callus cultures as compared to natural bulbs of onion. These results may be of pharmaceutical significance since the callus can be used as an alternative source for the isolation of antidiabe...

Crassocephalum rubens is a wild, economic and medicinal plant that is now threatened by genetic erosion in Nigerian ecosystem. The scarcity of the plant is due to indiscriminate collection from the wild and the difficulty in germinating the seeds. This study provides protocols for in-vitro propagation of C. rubens to supplement natural propagation, contribute to conserve the plant genetic resou...

Genotypic variation in cytokinin oxidase has been detected in enzyme preparations from Phaseolus vulgaris L. cv Great Northern and Phaseolus lunatus L. cv Kingston callus cultures. Although cytokinin oxidase preparations from Great Northern and Kingston callus tissues appear to have very similar substrate specificities, the cytokinin oxidase activities from the two callus tissues were found to ...