Discrete m-reps use tolerance-based, sampled medial skeleta as an underlying framework for boundaries defined by displaced subdivision surfaces. They provide local and global shape information, combining the strengths of multiscale skeletal modeling with the multi-resolution, deformation and shading properties afforded by subdivision surfaces. Hierarchically linked medial figures allow figural,...

A 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis, was identified, cloned, and sequenced. This fragment contains cis elements, including iterons, found in replication origins of other large plasmids and suggests that pBtoxis replicates by a type A theta mechanism. Two genes, pBt156 and pB...

Sixteen novel circular rep-encoding DNA sequences with high sequence homologies to previously described SPHINX and BMMF were isolated for the first time from non-bovine foods (pork, wild boar, chicken meat, Alaska pollock, pangasius, black tiger shrimp, apple, carrot, sprouts alfalfa, radish, broccoli). The phylogenetic analysis of full-length genomes grouped these together representatives SPHI...

Aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylases (16S RMTase) are two main resistance mechanisms against aminoglycosides. This study aimed to evaluate the frequency of AMEs methylase genes among aminoglycoside non-susceptible Acinetobacter baumannii isolates assess their clonal relationship using repetitive extragenic palindromic-PCR (rep-PCR). In this cross-sectional study, a to...

The massive development of virtualized infrastructures, Internet Things (IoT), and Wireless Sensor Network (WSN) in recent years has led to an increase quality requirements for the management reliability underlay communication networks. Existing converged networks must therefore guarantee specific quantitative qualitative parameters different network services meet customer requirements. However...

Integration into a particular location in human chromosomes is a unique property of the adeno-associated virus (AAV). This reaction requires the viral Rep protein and AAV origin sequences. To understand how Rep recognizes DNA, we have determined the structures of the Rep endonuclease domain separately complexed with two DNA substrates: the Rep binding site within the viral inverted terminal rep...

Abstract In this study the isogeometric B-Rep mortar-based mapping method for geometry models stemming directly from Computer-Aided Design (CAD) is systematically augmented and applied to partitioned Fluid-Structure Interaction (FSI) simulations. Thus, newly proposed methodology geometries described by their Boundary Representation (B-Rep) in terms of trimmed multipatch Non-Uniform Rational B-S...

Replication arrests due to the lack or the inhibition of replicative helicases are processed by recombination proteins. Consequently, cells deficient in the Rep helicase, in which replication pauses are frequent, require the RecBCD recombination complex for growth. rep recA mutants are viable and display no growth defect at 37 or 42 degrees C. The putative role of chaperone proteins in rep and ...

REPs are highly repeated intergenic palindromic sequences often clustered into structures called BIMEs including two individual REPs separated by short linker of variable length. They play a variety of key roles in the cell. REPs also resemble the sub-terminal hairpins of the atypical IS200/605 family of insertion sequences which encode Y1 transposases (TnpA(IS200/IS605)). These belong to the H...