AIM To present the problem of brucellosis caused by B. melitensis in Israel and to develop a rationalized control programme and eradication campaign in small ruminants. METHODS CFT, an OIE prescribed test and a confirmatory method, was used as a confirmatory test in support of legislation to compensate the farmers following a stamping-out policy. Positive reactors were cultured for Brucella s...

BACKGROUND Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. The major cause of brucellosis worldwide is brucella melitensis. Medicinal plants are considered as new antibacterial sources that could replace conventional antibiotics in the treatment of antibiotic-resistant bacteria. The aim of this study was to evaluate the efficacy of some native plants, alone and...

Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antig...

Brucellosis in goats is mainly caused by B. melitensis, although this pathogen may also infect cattle and other ruminants [1]. This pathogen has three different biovars [2]. B. melitensis was the first species in the genus Brucella described and was first isolated by Bruce in 1887 [3] from the spleens of soldiers dying of Mediterranean fever on the island of Malta. The origin of the disease rem...

Two hundred and fifty five biological samples were collected from 188 animals (81 sheep and 107 goats) during the lambing season from September 2009 to April 2010 from the Mafraq region of Jordan. Sampled animals belonged to 93 sheep and goat flocks that had abortion cases in the region. One hundred and seven (41.9%) biological samples were positive for the omp2 primers that were able to identi...

Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 20, No. 9, September 2014 1571 Although persistence of B. melitensis in wild ruminants has not been reported, and these animals are considered an epidemiologic dead-end reservoir (3), the unexpected prevalence observed (≈50%) suggests that Alpine ibex could be the source of bovine brucellosis reemergence in the study area in France. Strict s...

Brucella melitensis, as a pathogenic gram-negative intracellular bacterium, causes brucellosis in animals and humans. According to literature, the B. melitensis outer membrane protein 31 (Omp31) is considered as an important vaccine candidate against brucellosis. The aim of the current study was to compare three different expression constructs containing B. melitensis Omp31 antigen using bioinf...

Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent assay (ELISA) with polyclonal and protein G conjugates and smooth lipopolysacchar...

BACKGROUND Brucellosis remains a major worldwide zoonosis. Caprine brucellosis is a significant problem for both public health and animal production. Brucella melitensis causes disease in goats, sheep, humans, and occasionally cattle. Transmission is by ingestion or contact with infected materials, vaginal discharge, or milk. OBJECTIVES The current study aimed to determine the rate of B. meli...

Brucellosis is an infectious disease with high impact on innate immune responses which is induced partly by its DNA. In the present study the potential differences of wild type and patients isolates versus attenuated vaccine strains in terms of cytokines, ROS and NO induction on murine splenocytes and peritoneal macrophages were investigated. This panel varied in base composition and included D...