The use of deuterium-hydrogen exchange as a means of studying the folded structures of proteins is based upon the following observations (1). (a) Very rapid exchange takes place between solvent and exchangeable hydrogen atoms of simple amides (2), small peptides (3), polypeptides in random chain conformations (4), and denatured proteins, such as oxidized ribonuclease (5). Exchangeable hydrogens...

Ligand-binding properties of β-lactoglobulin (β-lg) are well documented, but the subsequent biological functions are still unclear. Focusing on fatty acids/β-lg complexes, the structure-function relationships are reviewed in the light of the structural state of the protein (native versus non-native aggregated proteins). After a brief description of β-lg native structure, the review takes an int...

Stability, aggregation and gelation of β-Lactoglobulin are affected by high pressure and salts of the Hofmeister series. Little is known about their combined effects on structure formation processes of β-Lactoglobulin, mainly because many salts of the series are not suitable for use in food. Here, we investigate the effect of calcium salts on the strength of pressure-induced gels, inspired by t...

In this study, it was aimed to investigate α -S1-casein and β -lactoglobulin levels some biochemical mineral values in raw milk samples obtained from cows, buffaloes, sheep, goats raised Samsun. The study material consisted of 40 taken goats, sheep buffaloes after milking a farm Samsun province Bafra district. α-S1-casein β-lactoglobulin serum were determined using ELISA test kits. Calcium (Ca)...

Lactoferrin (LF) and β-lactoglobulin (BLG) are among the protein pairs that exhibit heteroprotein coacervation, a unique and relatively unexamined type of liquid-liquid phase separation (LLPS). In prior work we found that LF and BLG undergo coacervation at highly constrained conditions of pH, ionic strength and protein stoichiometry. The molar stoichiometry in coacervate and supernatant is LF :...

Objective: To investigate the humoral and cellular immune response against cow’s milk proteins in Behçet’s disease and to distinguish any behaviour during active or inactive disease. Methods: Peripheral blood mononuclear cells from 16 patients and from eight normal controls were cultured in the presence of phytohaemagglutinin (PHA), β-casein, β-lactoglobulin, or chicken egg albumin. Interferon ...

Protein glycation (non-enzymatic glycosylation) and the development of advanced glycation end-products (AGEs) is strongly associated with the pathogenesis of diabetic secondary complications [I ] . Hydroxyl radicals generated from superoxide via the Fenton reaction have been implicated for in virro AGE formation [2]. The term glycoxidation has been coined to emphasise the involvement of metal c...

Nutritional quality of raw donkey milk (DM) may be impaired during sanitization with the current batch holder pasteurization systems (62.5 °C for 30 min). In this paper, we present preliminary results concerning effects high temperature short time (HTST) protocols using an innovative in continuous low flow rate plant (60 dm3/h) on B-vitamins group, antioxidant capacity, lysozyme and β-lactoglob...

The authors presented the haematological blood parameters of Romanov breed sheep and their association with genotypes according to β-lactoglobulin locus. Blood plays a significant role in body as metabolism is carried out. It delivers nutrients oxygen cells organs removes metabolic waste products carbon dioxide. intensity processes can be judged from data. Since enzymes, activity, levels, bioch...