In this study, we describe the characterization of microbial Transglutaminase -modified casein using a combination of immunological and proteomic techniques. Using 5-(biotinamido)pentylamine as an acyl acceptor probe, three Q residues of β-casein and one of αs1-casein were found to participate as acyl donors. However, no Q-residues were involved in network formation with κ-casein or αs2-casein....

Protein profile, in vitro gastrointestinal digestion and antioxidant potential of two fresh cheese samples obtained by kombucha inoculum traditional starter culture have been investigated this study. There is a significant difference protein profile bioactive between samples. Kombucha contains higher proportion protein, predominantly the casein fractions (α-casein β-casein) compared to culture....

In this study, we report differences in the observed gliding speed of microtubules dependent on the choice of bovine casein used as a surface passivator. We observed differences in both speed and support of microtubules in each of the assays. Whole casein, comprised of α(s1), α(s2), β, and κ casein, supported motility and averaged speeds of 966±7 nm/s. Alpha casein can be purchased as a combina...

A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.

To characterize the hypercholesterolaemic effect of casein further, four groups of young male rabbits in two separate experiments were placed on cholesterol-free semi-purified diets for 12 weeks. The diets were similar in composition, with either casein or soya-bean-protein isolate providing the protein source (250 g/kg). In two of these diets the salt mix was reduced by 45% (normally 40 g/kg) ...

Abstract Casein as the major protein of milk is a promising source for biopolymer fibers. Current casein-based fibers are fabricated by dissolving caseins in alkaline media and wet spinning coagulation bath containing harsh chemicals. In milk, casein present so-called micelles (CMs). Based on rennet-induced aggregation, we developed process that can be applied micellar sustainable way without u...

Adult male rabbits were fed on semi-purified diets containing soya-bean protein isolate, casein or formaldehyde-treated casein as the protein source and 1 g cholesterol and 5 g of the non-absorbable marker chronic oxide/kg diet. The concentration of cholesterol in serum and in liver was increased on both the casein and formaldehyde-treated-casein diets. Excretion of bile acids and their concent...

VÍTKOVÁ M., RAUCH P., FUKAL L. (2002): Optimisation of indirect competitive ELISAs of α-, β-, and κ-caseins for the recognition of thermal and proteolytic treatment of milk and milk products. Czech J. Food Sci., 20: 53–62. Polyclonal antibodies were raised against six immunogens (three native and three thermally treated casein fractions: α+β-casein, κ-casein and whole casein). Using these antib...

The effects of different sources of carbon, nitrogen and some of trace elements on cultivation of Bacillus thuringiensis H14 were studied. Glucose, glycerol, sodium acetate, (NH4)2SO4, corn steep liquor, yeast extract, hydrolyzed casein, Ca2+, Mg2+ and Mn2+ were the main parameters evaluated by Plackett-Burman statistical method. All el...

protein aggregation and precipitation is associated with many debilitating diseases including alzheimer's, parkinson's, and light-chain amyloidosis. β-casein, a member of the casein family, has been demonstrated to exhibit chaperone-like activity to protect protein form aggregation. hofmeister salts (lyotropice series) are a class of ions which have an effect on the solubility and also the stab...