AIM To determine whether the following can be sterilized by autoclaving - endodontic sponges, rotary nickel-titanium (NiTi) instruments within endodontic sponges, and rotary NiTi instruments with rubber stoppers. METHODOLOGY Sixty-four samples of eight different endodontic sponges (n = 512) were placed into brain heart infusion broth (BHI) for 72 h. An aliquot of this was then spread onto hor...

An experiment was conducted to compare two common methods of estimating bone ash from growing broiler chicks (A = autoclaving; B = boiling/extracting). Ross x Ross 1-d-old broiler chicks were fed a corn-soy, phosphorus-deficient diet (22.7% CP, 1% calcium, 0.22% non-phytate phosphorus), with 0, 750, 1,500, 3,000, or 6,000 units of phytase (FTU) to produce bones with a range of ash. The methods ...

The isolation of N10 formylfolic acid' from natural materials has been reported by several groups of workers (Rauen et al., 1952; Rauen and Jaenicke, 1953; Greenberg, 1954a; Silverman et al., 1954). It was concluded that the compound had been derived either from the degradation of a labile intermediate involved in the conversion of a bound (microbiologically inactive) form of folic acid in live...

Blood agar, prepared with Trypticase (BBL Microbiology Systems, Cockeysville, Md.) soy agar and 5% defibrinated bovine blood, is used for testing the potency of live Pasteurella multocida and Pasteurella haemolytica vaccines, but its potential for variation makes it undesirable to use in a standard assay method. Tests done with RPMI 1640 and Trypticase soy medium indicated that the benefits obt...

this study was conducted to investigate the effect of physical and chemical processing on crude protein fractions of two varieties of canola (okapi and modena) and safflower (62 and zarfan) seed using cornel net carbohydrate and protein system and on protein subunits using sodium dodecyl sulfate polyacrylamide gel electrophoresis. formaldehyde was used as chemical treatment, while toasting and ...

Forty crossbred neonatal pigs with an average initial age of 4 d and BW of 2.16 kg were used in a 28-d experiment to evaluate the nutritional effects of autoclaving a commercial sugar-free, spray-dried egg albumen (EA) compared with casein. Basal diet protein sources were lactic acid casein and EA. Two more dietary treatments were made by replacing the EA with dry-autoclaved EA (DAEA) or wet-au...