A membrane fraction enriched in endoplasmic reticulum was prepared from rat parotid glands by using sucrose-gradient centrifugation. The fraction showed a 10-fold increase in specific activity of NADPH: cytochrome c reductase activity over that of tissue homogenates and minimal contamination with plasma membranes or mitochondria. The endoplasmic reticulum fraction possessed both Mg2+ -stimulate...

Aldosterone-induced intestinal Na(+) absorption is mediated by increased activities of apical membrane Na(+)/H(+) exchange (aNHE3) and basolateral membrane Na(+)-K(+)-ATPase (BLM-Na(+)-K(+)-ATPase) activities. Because the processes coordinating these events were not well understood, we investigated human intestinal Caco-2BBE cells where aldosterone increases within 2-4 h of aNHE3 and alpha-subu...

The aim of the work is to study pharmacological substances that play a role eNOS expression regulators in modification lead intoxication effects experiment. Materials and methods. In experiment, linear male rats same age were used: intact with (120 heads). design was following: group 1 – control; 2 acetate solution; 3 + L-nitroarginine methyl ester; 4 5 L-arginine; 6 L-arginine. research carrie...

The purpose of this study was to investigate the protective effect Beta vulgaris leaf extract (BVLE) on Fe2+-induced oxidative testicular damage via experimental and computational models. Oxidative induced incubation tissue supernatant with 0.1 mM FeSO4 for 30 min at 37 °C. Treatment achieved by incubating tissues BVLE under same conditions. catalase (CAT), superoxide dismutase (SOD), glutathio...

H+ and Mga+ are known to inhibit Ca2+ binding to the transport sites of sarcoplasmic reticulum-ATPase. Evaluation of the affinity for the Ca2+ binding sites requires measurement of the amount of Ca2+ bound to ATPase as a function of the free Ca" concentration imposed by a Ca2+ chelator. The choice of the chelator is crucial as it determines the accuracy of the free Ca2+ concentration. At pH > ...

The role of the NH2-terminal domain of the 20,000-dalton light chain on the regulatory function of smooth muscle myosin was studied by exchanging it in myosin with various mutant forms. The 10 S to 6 S conformational transition as well as the thick filament formation were significantly influenced by the deletion or substitution of the amino acid residues at the NH2-terminal side of the phosphor...

Patients with chronic renal failure frequently develop cardiac hypertrophy and diastolic dysfunction; however, the mechanisms by which this occurs are still unclear. Male Sprague-Dawley rats were subjected to 5/6 nephrectomy and studied for their isolated myocyte function, calcium cycling, and gene expression of proteins important in calcium homeostasis after 4 wk. Comparable rats subjected to ...

The extrusion of protons is considered a very general parameter of the activation of many kinds of membrane or intracellular molecules, such as receptors, ion channels, and enzymes. We found that pepstatin A caused a reproducible, concentration-related increase in the extracellular acidification rate in two microglial cell lines, Ra2 and 6-3. Washing abolished pepstatin A-induced acidification ...

background: this study was aimed to assess the effects of angiotensin ii (ang ii) supplementation to the in vitro maturation (ivm) and in vitro culture (ivc) media of vitrified-warmed ovine oocytes on their developmental competence and expression of na+/k+/atpase in resulting embryos. methods: the slaughterhouse-derived immature oocytes (n=1069) were randomly distributed into four experimental ...