Enzyme cascades are an emerging synthetic tool for the synthesis of various molecules, combining advantages biocatalysis and one-pot multi-step reactions. However, more complex enzyme cascade is, difficult it is to achieve adequate productivities product concentrations. Therefore, whole process must be optimized account synergistic effects. One way deal with this challenge involves data-driven ...

Background: The mitochondria are an important source of adenosine triphosphate (ATP) production in pre-implantation embryo. Therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and ATP content. Methods: The embryos at 2-PN, 4-cell and blastocyst stages were collected from the oviduct of st...

Nano-zero-valent iron (nZVI) can be used to remove nitrate nitrogen (NO3-N) from groundwater. However, it has low reduction efficiency owing its oxidation and aggregation characteristics. Thus, nZVI-loaded material is alleviate these drawbacks. In this study, nZVI-coated attapulgite (ATP) was prepared for the removal of NO3-N ATP-nZVI using chemical liquid deposition-coreduction method. The mat...

Khalatbary AR1, Tarihi T2, Naderimanesh H3 1. Assistant Professor, Department of Anatomy, Faculty of Medicine, Lorestan University of Medical Sciences 2. Professor, Department of Anatomy, Faculty of Medicine, Ttarbiat Modarres University , Tehran 3. Professor, Department of Biophysics, Faculty of Basic Sciences, Tarbiat Modarres University , Tehran Abstract Background: Apoptosis is an active ...

Guanosine 5’-diphospho-β-l-fucose (GDP-Fuc) is an important and expensive nucleotide sugar only available in low quantities. We established two processes for the gram-scale production of GDP-Fuc with multi-enzyme cascades. used bifunctional enzyme fucokinase/GDP-fucose pyrophosphorylase from Bacteroides fragilis either ATP-regeneration system using polyphosphate or excess ATP. The optimized mod...

This study aims to analyse Tunisian farmers’ ability pay (ATP) in a citrus area and propose penalising price strategy based on the block-pricing process decrease over-irrigation without affecting incomes. The methodology is residual imputation approach determine ATP, stochastic production frontier estimate technical efficiency optimal water irrigation quantity calculation of elasticity demand f...