A RP-HPLC assay method has been developed and validated for cefoperazone. An isocratic RP-HPLC was developed on a SS Wakosil IIC18 column (250 mm × 4.6 mm i.d., 5 μm) using a mobile phase of phosphate buffer (pH 6.8) and methanol (3:1 v/v) with UV detection at wavelength 254 nm at the flow rate 1 ml/min. The proposed method was validated for sensitivity, selectivity, linearity, accuracy, precis...

A simple, sensitive and precise RP-HPLC method was developed for the determination of dutasteride in tablet dosage form. The RP-HPLC separation was achieved on phenomenex C(18) column (250 mm, id 4.6 mm, 5 mum) using mobile phase methanol:water (90:10 v/v) at a flow rate of 1 ml/min at an ambient temperature. Quantification was achieved with photodiode array detection at 235 nm over the concent...

Determining to what extent biophysical characteristics of aggregates affect immunogenicity of therapeutic interferon beta-1b. Three recombinant human interferon beta-1b (rhIFNβ-1b) samples with different levels of aggregates generated by copper oxidation, thermal stress, or left untreated, as well as Avonex(®) drug substance and Betaferon(®) drug product, were injected intraperitoneally in nont...

Objective: The present work was undertaken with the aim to develop and validate a rapid and consistent RP-HPLC method in which the peaks will be appear with short period of time as per ICH Guidelines. Methods: The proposed method was simple, fast, accurate and precise method for the Quantification of drug in the dosage form, bulk drug as well as for routine analysis in Quality control. Reversed...

To determine the safety and efficacy, we have developed validated RP-HPLC method for simultaneous quantification of cinnarizine, its five specified impurities two degradation products in cinnarizine API, tablets capsules including antioxidants an oral suspension formulation. The chromatographic separation was achieved gradient elution mode with 1.00mL/min flow on Ascentis Express C18 (150mm, 4....

A simple, sensitive, and reproducible reversed-phase high-performance liquid chromatography (RP-HPLC) method, coupled with a photodiode array detector, was developed for the determination of rupatadine (RUPA) and its related substances in pharmaceutical dosage forms. Chromatographic separation was achieved on the Hypersil BDS (150 x 4.6 mm, 5 μm) column with a mobile phase containing a gradient...

Chamomile is a medicinal plant, which presents several biological effects, especially the anti-inflammatory effect. One of the compounds related to this effect is apigenin, a flavonoid that is mostly found in its glycosylated form, apigenin-7-glucoside (APG), in natural sources. However, the affectivity and safety of this glycoside have not been well explored for topical application. In this co...

Juvenile hormone (JH) biosynthesis and the effects of synthetic Manduca sexta allatostatin (Mas-AS) and M. sexta allatotropin (Mas-AT) were investigated in isolated corpora allata (CA) of Vth stadium larvae of the tomato moth, Lacanobia oleracea. Reversed-phase high-performance liquid chromatography (RP-HPLC) of JH extracted from CA shows that larvae produce predominantly JH II and its correspo...

The main aim of present work was to develop a simple, precise, rapid and reproducible isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of Imatinib in pure and in tablet dosage form. An isocratic RP-HPLC was performed by utilizing Welchrom C18 column, (250 mm × 4.6 mm i.d., particle size 5 μm) maintained at ambient temperature and mobile phase c...

Purpose: To evaluate the total phenolic content and antioxidant potential of the methanol extracts of aerial parts and roots of Thymus sipyleus Boiss and also to determine some phenolic compounds using a newly developed and validated reversed phase high performance liquid chromatography (RP-HPLC) method. Methods: The total phenolic concentration of the extracts were determined using Folin Cioca...