A yeast gene, DDI1, has been identified as a suppressor of a temperature sensitive mutant of the PDS1 gene, which encodes Pds1p (Securin), a key regulator of the cell cycle whose ubiquitin-dependent proteolysis initiates anaphase in budding yeast [1–3]. The Ddi1 protein has, at its carboxyl terminus, a ubiquitin associated domain that is required for the suppression of Pds1 mutations and has be...

This study include isolation of some types Aspergillus sp from different sources such as ( soil, vegetables), three appeared; fumegates , nidulans and ochracaeus ,then  tested the virulence factors these fungi production enzymes (proteinase ,phospholipase ,heamolycin ,lipase urease ) give positive result for of  phospholipase  ,lipase urease; enzyme proteinase product only A. .al...

Activity of the lactococcal cell envelope-located serine proteinase depends on the presence of membrane-associated lipoprotein PrtM. To differentiate between the action of the proteinase and the action of PrtM in the process of proteinase maturation, an inactive form of the lactococcal proteinase was constructed. This was done by mutating one of the three amino acids thought to constitute the a...

The Streptococcus cremoris Wg2 proteinase gene, cloned in S. lactis, specified a proteinase which exhibited the same specificity toward casein as did the proteinase isolated from the original host. Although the cloned gene lacked the last 130 codons, the proteinase still specifically degraded beta-casein. Deletion of the C-terminal 343 amino acids from the proteinase did not influence this spec...

A supernatant fraction was prepared from rat uterine myometrium by homogenization, sonication and centrifugation. In this supernatant the protein concentration and the activities of an acid proteinase, an acid phosphatase and a proteinase inhibitor were measured. From the fibrous sediment, after washing with 0.5% Triton X-100 and with water, an actomyosin-containing solution was obtained by ext...

We have identified and characterized three stage-specific proteinases of Plasmodium falciparum that are active at neutral pH. We analyzed ring-, trophozoite-, schizont-, and merozoite-stage parasites by gelatin substrate PAGE and characterized the identified proteinases with class-specific proteinase inhibitors. No proteinase activity was detected with rings. Trophozoites had a 28 kD proteinase...

Inflammatory mouse peritoneal macrophages secrete a metalloproteinase that is not inhibited by alpha 1-proteinase inhibitor. This proteinase, macrophage elastase, recognizes alpha 1-proteinase inhibitor with macrophage elastase does not involve a stable proteinase-inhibitor complex and results in the proteolytic removal of a peptide of apparent molecular weight 4,000-5,000 from the inhibitor. A...

Comparative studies of aspartyl-, leucyl-, peripheral blood of a patient with miolseryland tyrosyl-tRNA by a reverse tiple myeloma and produce 1gM and phase column co-chromatography have lambda-type light chain (Bence Jones been performed between a human protein), respectively. Similar chromanormal lymphoblast cell line (RFMI tographic patterns were obtained for 1788) and a human myeloma cell l...