We determined the effects of mono-L-arginine-containing compounds on pial arterioles of anesthetized piglets. A closed cranial window was implanted, and the diameter of one pial arteriole was determined by intravital microscopy. Diameter was determined during application of artificial cerebrospinal fluid containing no drugs and during application of 10-5 10-4, 10-3, and 10-2 M L-arginine (ARG),...

1. In previous studies we have shown that, after the administration of adriamycin, hypertension developed in rats who became pregnant (adriamycin-pregnant rats), whereas virgin animals remained normotensive. Subsequently, we showed that this hypertension was prevented by administration of L-arginine, suggesting that deficient synthesis of nitric oxide may be pathogenetic in this model. 2. To fu...

Nitric oxide synthase (NOS) was evidenced in mature mouse spermatozoa by means of biochemical techniques and Western blot. During 120 min of incubation, 10(7) spermatozoa synthesized 7 +/- 2 pmol of L-[14C]citrulline. Besides, L-citrulline formation depended on the incubation time and on the concentration of L-arginine present in the incubation medium. Different concentrations of N(G)-nitro-L-a...

the fruit of melia azedarach l. (meliaceae) was extracted with hexane, and its fatty acids methyl ester was analyzed using gc/ms. thirteen components representing 85.1% of the total extract were identified. the major components identified were methyl palmitate (18.8%), methyl linolenate (16.1%) and methyl linoleate (9.8%). the fruits’ extract had a lc50 of 5.5 ppm against the larvae of anophles...

BACKGROUND Escherichia coli heat stable enterotoxin (STa) is a major cause of secretory diarrhoea in humans. AIMS To assess the effects of instilling STa into the ileum on remote fluid secretion in the jejunum and colon in rats in vivo by a gravimetric technique. METHODS AND RESULTS Ileal STa (55 ng/ml) stimulated fluid secretion in both ileal and jejunal loops but not in the colon. The flu...