Although mice are commonly used for studies of atherosclerosis, investigators have had no convenient way to quantify apolipoprotein (apo) B, the major protein of atherogenic lipoproteins, in this model. We now report an automated immunoturbidimetric assay for mouse apo B with an NCCLS imprecision study CV < 5%. Added hemoglobin up to 50 g/L did not interfere with the assay, nor did one freeze-t...

Liu, Min, Ling Shen, Yin Liu, Stephen C. Woods, Randy J. Seeley, David D’Alessio, and Patrick Tso. Obesity induced by a high-fat diet downregulates apolipoprotein A-IV gene expression in rat hypothalamus. Am J Physiol Endocrinol Metab 287: E366–E370, 2004. First published April 13, 2004; 10.1152/ajpendo.00448.2003.— Apolipoprotein A-IV (apo A-IV) is an anorectic protein produced in the intestin...

Apolipoprotein A-l-contalnlng llpoprotelns (high density llpoproteins, HDL) can be separated Into two subtractions, which have pre-beta and alpha electrophoretic mobilities, respectively. These fractions differ In both composition and structure. Some preparations of pre-beta-mlgratlng HDL, but not alpha-migrating HDL, were found to contain two polypeptldes with Mr of approximately 26 and 14 kDa...

The intensity of scattered light varies with the size of the scattering particles. Studying the quantitative immunonephelometric determination of apolipoprotein A-1 (apo A-1), we observed that the different particle sizes of lipoproteins must be taken into account in immunonephelometry if apo A-1 is a constituent of the lipoprotein particles. Because interaction between large lipoproteins and i...

We describe an isoelectric-focusing method for rapidly separating and quantifying apolipoprotein (apo) C-II and C-III subspecies of triglyceride-rich lipoproteins. Concentrated very-low-density lipoproteins (VLDL) or delipidated apo VLDL are focused on dry acrylamide plates, after their rehydration with ampholytes and urea. Apo C-II and apo C-III in VLDL are resolved into four major bands--C-II...

INTRODUCTION Polymorphisms in the cholesterol ester transfer protein (CETP) gene and apolipoprotein AI (apo AI) gene are identified as the most common genetic factors influencing high-density lipoprotein cholesterol (HDL cholesterol) levels. Low HDL cholesterol is an important risk factor for cardiovascular disease. We investigated the effect of the TaqIB polymorphism of the CETP gene and the 7...

Long-chain polyunsaturated fatty acids (LC-PUFA) are important in the development of the immature nervous system, and adding these fatty acids to infant formula has been proposed. To determine the effect of n-3 LC-PUFA on apolipoprotein secretion and lipid synthesis in newborn swine enterocytes, differentiated IPEC-1 cells were incubated for 24 h with docosahexaenoic acid (DHA; 22:6) or eicosap...

Background: Coronary heart disease (CHD) is mainly related to hypercholesterolemia. Sometimes CHD occurs in people with normal cholesterol. Therefore, it necessary study other factors that cause CHD: apolipoprotein B (apo-B). Atherosclerosis a marker of CHD, characterized by the thickening walls blood vessels and narrowing vessels. Non-pharmacologically, can be managed consuming foods high fibe...

Abstract Introduction The clinical consequences of atherosclerosis and post-procedure neointimal hyperplasia (NIH) represent a significant proportion the vascular surgeon's workload. Lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) is class E scavenger receptor expressed in endothelial cells implicated NIH. This study aims to demonstrate therapeutic potential LOX-1 targeting usin...

We have produced five hybridomas which secreted monoclonal antibodies that reacted with human plasma apolipoprotein D. On analysis by polyacrylamide gel electrophoresis (PAGE) high density lipoproteins and lecithin:cholesterol acyltransferase (EC 2.3.1.43)-enriched fractions of plasma contained many protein bands that reacted with the antibodies. Purified apolipoprotein D had the lowest Mr (29,...