Human beta-defensin (hBD)-3, a 45 amino acid antimicrobial peptide, was originally isolated from human skin. hBD-3 mRNA has also been detected in the airways by RT-PCR. While hBD-3 may be involved in antimicrobial defences within the respiratory tract, the presence of hBD-3 peptide in the respiratory system has not yet been confirmed. The antimicrobial activity of the synthesised hBD-3 peptide ...

Nature is an unexplored reservoir of novel phytopharmaceuticals. Since biofilm-related oral diseases often correlate with antibiotic resistance, plant-derived antimicrobial agents could enhance existing treatment options. Therefore, the rationale of the present report was to examine the antimicrobial impact of Mediterranean natural extracts on oral microorganisms. Five different extracts from O...

The presence of aminoglycoside antibiotics interferes with the determination of clindamycin levels by standard microbiological assay. A new bioassay method of quantitation of clindamycin in the presence of aminoglycosides is presented. This technique is based on the fact that incorporation of calcium chloride into assay agar blocks the antimicrobial activity of aminoglycosides against Sarcina l...

Chitosan is a naturally occurring polysaccharide with antimicrobial properties. This study investigated whether chitosan could induce the LytSR two-component autolytic regulatory system, specifically the cid and lrg operons, found in Streptococcus mutans. A turbidity assay and a murein hydrolase assay were used to observe autolytic activity in chitosan-treated and non-treated cultures of wildty...

Since ancient time, Phyllunthus niruri L. are using in many herbal preparations. Increasing demand of P. niruri lead to adulteration, and thus there is a global need to develop quality plant material. In present investigation, an efficient in vitro technology was developed for a rapid and large-scale production of P. niruri via axillary bud proliferation using nodal segments of the mature plant...

Based on the traditional usage, Moringa oleifera and Allamanda cathartica plants were selected. The present study is to find out its antimicrobial potential against clinical isolates of KMCHospital. The resistant patterns of the isolated pathogens were studied against commercial standard antibiotics. By using the different polar solvents (Methanol, Ethanol, Chloroform, Water, Petroleum ether an...

BACKGROUND It has been recently recognized that oxidative stress, helminth and microbial infections are the cause of much illness found in the underdeveloped, developing and developed countries. The present study was undertaken to identify the chemical composition, and to assess anthelmintic, antimicrobial and antioxidant effects of Thymus bovei essential oil. METHODS The chemical composition...

Cytotoxic and antimicrobial effects of Montivipera xanthina venom against LNCaP, MCF-7, HT-29, Saos-2, Hep3B, Vero cells and antimicrobial activity against selected bacterial and fungal species: Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, E. coli O157H7, Enterococcus faecalis 29212, Enterococcus faecium DSM 13590, Staphylococcus epidermidis ATCC 12228, S. typhimirium CCM 5445...

Cultured epidermal skin has become an adjunctive therapy for treatment of major burn injuries, but its effectiveness is greatly limited because of destruction by microbial contamination. To evaluate candidate antimicrobial agents for use with cultured skin, a combined cytotoxicity-antimicrobial assay system was developed for determination of toxicity to cultured human keratinocytes and fibrobla...

Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends to explore the role of interaction pattern at the iron oxide nanoparticle (IONP)-bacteria interface affecting antimicrobial propensity of IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IO...