Many pathogenic fungi may be recognized grossly by their appearance in culture. The more definitive method of identification, however, is through microscopic examination of the structures on which classification depends: the spores and the sporebearing apparatus. The simplest method of accomplishing this is to tease a small fragment of mycelium out of the culture and crush it under a cover slip...

Fluorescence behavior of 8-anilino-1-naphthalenesulfonate (ANS) reflects a blue-shift and fluorescence enhancement on decreasing solvent polarity, with both properties affected in a synchronous way in solvent mixtures where ANS senses a homogeneous solvation shell. ANS complexation by cyclodextrins or bovine serum albumin (BSA) results in a nonhomogeneous solvation shell that is reflected by no...

Chemicals-o-Aminophenyl glucosiduronic acid was kindly supplied by Dr. R. T. Williams, St. Mary’s Hospital Medical School, London. oAminobenzoyl and p-aminobenzoyl glucosiduronic acids were prepared enzymatically with UDP-glucuronic acid’ and aglycone (5). Phenolphthalein glucosiduronic acid and bacterial P-glucuronidase were obtained from the Sigma Chemical Company, St. Louis, Missouri. UDP-gl...

The title compound, C(8)H(8)N(2)O(7)S, an inter-mediate in the synthesis of N,N-bis-(2-hydroxy-ethyl)-3,5-dinitro-aniline, exists as a discrete mol-ecule; the nitro groups are twisted with respect to the aromatic system [dihedral angles = 17.0 (1) and 26.3 (1)°].

Follicular populations were investigated in female F344/N rats to better understand the aging process of the rat ovary. Ovaries dissected at various ages (spanning 1-36 months old) were submitted for histological examination. The total number of primordial, growing (primary and secondary), tertiary, and atretic follicles as well as corpora lutea (CL) were counted in hematoxylin-eosin- and azoca...

The impact of sperm DNA fragmentation on assisted reproductive technology (ART) successes, in terms of outcome, is now established. High levels of DNA strand breaks severely affect the probability of pregnancy. The importance of sperm nucleus condensation in early embryogenesis and, subsequently, on the quality of the conceptus is now emerging. In this article we have compared in situ analyses ...

Background: Acrylamid (AA) is found in carbohydraterich foods that are prepared at high temperatures, such as French fries and potato chips. The toxic effects of AA in male animals include degeneration of epithelial cells of seminiferous tubules, decreased number of sperm and elevation in abnormal spermatozoa. The aim of this study was to evaluate the detrimental effects of AA on sperm paramete...