It has become a general notion that there are multiple GnRH systems in the vertebrate brains. To measure GnRH release activities from different GnRH systems, we conducted a static incubation of brain-pituitary slices under various conditions, and GnRH released into the incubation medium was measured by RIA. The slices were divided into two parts, one containing GnRH neurons in the preoptic area...

Pyrazinamide (PZA) is a critical component of first- and second-line treatments of tuberculosis (TB), yet its mechanism of action largely remains an enigma. We carried out a genetic screen to isolate Mycobacterium bovis BCG mutants resistant to pyrazinoic acid (POA), the bioactive derivative of PZA, followed by whole genome sequencing of 26 POA resistant strains. Rather than finding mutations i...

in this work, poly (o-anisidine) (poa) was prepared by consecutive potential cycling in an acidic monomer solution at the surface of carbon paste electrode (cpe). nickel ions were dispersed into the polymer by immersing the electrode in niso4 solution. the prepared ni/poa/cpe was characterized by scanning electron microscopy, energy dispersive x-ray spectroscopy, atomic force microscopy (afm) a...

The fungus Acremonium typhinum produces a novel endoprotease during symbiotic endophytic infection of the grass, Poa ampla. This protease is unusual because it is highly active in the presence of sodium dodecyl sulfate. The enzyme is a thiol-containing serine protease and is localized to a crude membrane fraction. Similar protease activity has been detected in endophyte-infected Poa autumnalis ...

Allison, C.W. and Awramik, S.M., 1989. Organic-walled microfossils from earliest Cambrian or latest Proterozoic Tindir Group rocks, northwest Canada. Precambrian Res., 43: 253-294. Uppermost Tindir Group limestones exposed in the headwaters of Tindir Creek, Yukon Territory, Canada, contain a diverse and abundant microbiota of mineralized and organic-walled microfossils. Preserved primarily in c...

Recent advances in mass spectrometry (MS) technology have facilitated the detection and quantification of minor components in organisms and the environment. In this study, we successfully identified 20-hydroxyecdysone (20E) in first instar nymphs (7 days after hatching) of the scorpion Liocheles australasiae, using tandem mass spectrometry combined with high-performance liquid chromatography (L...

Fever is the widely known hallmark of disease and is induced by the action of the nervous system. It is generally accepted that prostaglandin (PG) E(2) is produced in response to immune signals and then acts on the preoptic area (POA), which triggers the stimulation of the sympathetic system, resulting in the production of fever. Actually, the EP3 subtype of PGE receptor, which is essential for...

This is the third column in our series explaining the new OMG Portable Object Adapter. An Object Adapter is the CORBA component responsible for adapting CORBA’s concept of objects to a programming language’s concept of servants. As you’ll recall from previous columns [1, 2], CORBA objects are “abstract” entities defined by IDL interfaces. Likewise, servants are “concrete” implementations of COR...